| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,358,400 | G→A | 16.4% | A362T (GCT→ACT) | glpA → | sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,358,400 | 0 | G | A | 16.4% | 205.1 / 27.1 | 79 | A362T (GCT→ACT) | glpA | sn‑glycerol‑3‑phosphate dehydrogenase (anaerobic), large subunit, FAD/NAD(P)‑binding |
| Reads supporting (aligned to +/- strand): ref base G (23/43); new base A (8/5); total (31/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGA > W3110S.gb/2358330‑2358464 | tGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATgg > 1:3680735/1‑71 (MQ=255) gCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGc > 1:1875400/1‑71 (MQ=255) aaCGCGATGGTCTGGACGTATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGa < 1:3224863/70‑1 (MQ=255) aCGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGaa > 1:1941297/1‑70 (MQ=255) aCGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAAt < 1:1509362/71‑1 (MQ=255) cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATg < 1:2469977/71‑1 (MQ=255) cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATg < 1:1304229/71‑1 (MQ=255) cgcgATGGTCTGGACGGATTTATCACCATCACCGGTGCCAAACTGATGACCTATCGGCTGATGGCTGAATg < 1:661099/71‑1 (MQ=255) gATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGc < 1:3947141/71‑1 (MQ=255) aTGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCt < 1:2203208/71‑1 (MQ=255) aTGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCt < 1:4445487/71‑1 (MQ=255) tGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGaa > 1:3888568/1‑64 (MQ=255) tGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCt > 1:406337/1‑70 (MQ=255) gTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTAcc < 1:92956/71‑1 (MQ=255) tCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCg < 1:1720895/71‑1 (MQ=255) tCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCg < 1:4432531/71‑1 (MQ=255) cGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCgg < 1:1533954/71‑1 (MQ=255) gATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTa < 1:3539173/71‑1 (MQ=255) aTTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTAt > 1:560484/1‑71 (MQ=255) tttATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATgg < 1:3253055/46‑1 (MQ=255) tttATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTa > 1:1659216/1‑69 (MQ=255) tttATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGCCTACCGACGCGGTATg < 1:2897965/71‑1 (MQ=255) ttATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATg < 1:4026034/70‑1 (MQ=255) tATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGc < 1:3961367/70‑1 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCg < 1:1311726/70‑1 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCg < 1:1623370/70‑1 (MQ=255) tcaccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCg < 1:1618242/70‑1 (MQ=255) caccatcaccGGTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGc > 1:4318420/1‑70 (MQ=255) accatcaccGGTGGCAAACTTATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCa < 1:2567167/70‑1 (MQ=255) accatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGc > 1:254534/1‑66 (MQ=255) accatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCa < 1:3870510/70‑1 (MQ=255) accatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCa < 1:4009497/70‑1 (MQ=255) accatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCa < 1:4127626/70‑1 (MQ=255) accatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCa < 1:3644225/70‑1 (MQ=255) accatcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCa < 1:3625105/70‑1 (MQ=255) accatcaccGGGGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCa < 1:4227446/70‑1 (MQ=255) catcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAAc < 1:3509871/71‑1 (MQ=255) atcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCaa < 1:568911/68‑1 (MQ=255) tcaccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCa < 1:262122/66‑1 (MQ=255) accGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTgg > 1:3048588/1‑70 (MQ=255) ccGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTgg < 1:4183120/69‑1 (MQ=255) ggTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCaa < 1:1950396/71‑1 (MQ=255) ggTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCaa < 1:1983225/71‑1 (MQ=255) gTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCgg < 1:2928690/51‑1 (MQ=255) gTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCaa > 1:797808/1‑70 (MQ=255) gTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCaa < 1:1720468/70‑1 (MQ=255) gTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAac < 1:368642/71‑1 (MQ=255) gTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCaa > 1:4236379/1‑70 (MQ=255) gTGGCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCaa > 1:3573796/1‑70 (MQ=255) gCAAACTGATGACCTATCGGCTGATGGCTGAATggg < 1:1778364/36‑1 (MQ=255) gCAAACTGATGACCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTggg > 1:1136124/1‑64 (MQ=255) cAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACAcgc < 1:4207905/71‑1 (MQ=255) aaCTGATGACCTATCGGCTGATTGCTGAATGGGCTACCGACGCGGTATGc < 1:2515308/50‑1 (MQ=255) aaCTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACgcgc > 1:2825553/1‑71 (MQ=255) aCTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTa > 1:1839529/1‑46 (MQ=255) aCTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGcc > 1:670550/1‑71 (MQ=255) aCTGATGACCTATCGGCTGATGGCTGAATGGGCTACAGACGCCGTATGCCGCAAACTGGGCAACACGCGcc > 1:2202949/1‑71 (MQ=255) gatgaCCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGcccc > 1:17379/1‑70 (MQ=255) atgaCCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTggg > 1:194115/1‑56 (MQ=255) atgaCCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTg > 1:504465/1‑71 (MQ=255) atgaCCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCAAAc > 1:915931/1‑52 (MQ=255) tgaCCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACAcgc > 1:2135588/1‑63 (MQ=255) tgaCCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGt < 1:3513890/71‑1 (MQ=255) gaCCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTa > 1:1627223/1‑71 (MQ=255) aCCTATCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTAc > 1:1372947/1‑71 (MQ=255) ccTATCGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACg < 1:4251739/71‑1 (MQ=255) cTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTg < 1:3371999/66‑1 (MQ=255) cTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGa > 1:4407990/1‑71 (MQ=255) cTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGa < 1:4455055/71‑1 (MQ=255) aTCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACt > 1:2614056/1‑71 (MQ=255) tCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTTGGCAACACGCGCCCCTGTACGACt < 1:1445567/70‑1 (MQ=255) tCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGAc < 1:4066775/69‑1 (MQ=255) tCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACt < 1:4611048/70‑1 (MQ=255) cGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAac < 1:604713/51‑1 (MQ=255) cGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCaaa > 1:3454110/1‑42 (MQ=255) cGGCTGATGACTGAATGGGCTACCGACGCGGTATGCCGCaaa > 1:2040623/1‑42 (MQ=255) ggCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGcc > 1:1678001/1‑71 (MQ=255) cTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGa < 1:1303060/71‑1 (MQ=255) tGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTAc < 1:4425397/61‑1 (MQ=255) aTGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACt > 1:4166496/1‑38 (MQ=255) aTGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACt > 1:4160959/1‑38 (MQ=255) ggCTGAATGGGCTACCGACGCGGTATGCCGCAAACt > 1:4480957/1‑36 (MQ=255) | TGCTGAACGCGATGGTCTGGACGGATTTATCACCATCACCGGTGGCAAACTGATGACCTATCGGCTGATGGCTGAATGGGCTACCGACGCGGTATGCCGCAAACTGGGCAACACGCGCCCCTGTACGACTGCCGA > W3110S.gb/2358330‑2358464 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |