| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,462,102 | A→G | 9.6% | F53L (TTC→CTC) | sixA ← | phosphohistidine phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,462,102 | 0 | A | G | 9.6% | 138.7 / 7.2 | 52 | F53L (TTC→CTC) | sixA | phosphohistidine phosphatase |
| Reads supporting (aligned to +/- strand): ref base A (20/27); new base G (2/3); total (22/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.01e-01 | |||||||||||
ACTTCTGCACTGGAAGGCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGA > W3110S.gb/2462035‑2462168 | aCTTCTGCACTGGAAGGCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACg < 1:3913573/70‑1 (MQ=255) cTTCTGCACTGGAAGGCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACggg < 1:2446052/71‑1 (MQ=255) cACTGGAAGGCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTc < 1:1470703/68‑1 (MQ=255) cACTGGAAGGCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTc < 1:2066408/68‑1 (MQ=255) cACTGGAAGGCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCa > 1:2785513/1‑69 (MQ=255) cACTGGAAGGCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCAc < 1:133742/70‑1 (MQ=255) aCTGGAAGGCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCAc > 1:1915373/1‑69 (MQ=255) tGGAAGGCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGaa > 1:560297/1‑58 (MQ=255) tGGAAGGCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAAc < 1:3308792/59‑1 (MQ=255) gAAGGCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAAc > 1:2081502/1‑71 (MQ=255) ggCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAAcgcg < 1:2852745/71‑1 (MQ=255) ggCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAAcgcg < 1:1650013/71‑1 (MQ=255) gCAGGTTCAGACAATCCCCTACTTCTTCTAGTGTTTGCTCGGATCGCAGGAACGGGCt > 1:151591/1‑58 (MQ=255) gCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAAc < 1:2590642/53‑1 (MQ=255) ggTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACg < 1:118091/51‑1 (MQ=255) gTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGaa > 1:2547885/1‑48 (MQ=255) gTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGa < 1:2508647/71‑1 (MQ=255) ttCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGAt < 1:315102/71‑1 (MQ=255) tCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCa > 1:2407252/1‑57 (MQ=255) tCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGaa > 1:4185591/1‑60 (MQ=255) tCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGa > 1:3647027/1‑69 (MQ=255) cAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAGCGGGCTCACCAGAACGCGTTCGAtt > 1:3373261/1‑70 (MQ=255) cAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGAttt > 1:2905200/1‑71 (MQ=255) cAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGAtt < 1:4465886/70‑1 (MQ=255) gACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACg < 1:676333/45‑1 (MQ=255) aCAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTc > 1:4270324/1‑69 (MQ=255) cAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCAc > 1:3275331/1‑71 (MQ=255) aaTCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACt < 1:3158449/71‑1 (MQ=255) tCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACttt > 1:353733/1‑71 (MQ=255) cccTACTTCTTCCAGTGTTTGCTCGGCTCTCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACtttt < 1:973434/70‑1 (MQ=255) cccTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAGCGGGCTCACCAGAACGCGTTCGATTTCCACttttt < 1:3467795/71‑1 (MQ=255) cTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAg < 1:4084623/46‑1 (MQ=255) tACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTc > 1:1878604/1‑54 (MQ=255) tACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGa > 1:3005269/1‑70 (MQ=255) cttcttCCAGTGTTTGCTCGGCTCGCAGGAGCGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGAcc < 1:4332385/70‑1 (MQ=255) ttcttcCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTc > 1:4462448/1‑57 (MQ=255) cttcCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCttt > 1:908294/1‑70 (MQ=255) cttcCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCttt < 1:3874238/70‑1 (MQ=255) cttcCAGTGTTTGCTCGGCTCGCAGGAACGGGATCACCAGAACGCGTTCGATTTc > 1:3774529/1‑55 (MQ=255) ccAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCttt > 1:2330754/1‑67 (MQ=255) ccAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAg < 1:3794744/70‑1 (MQ=255) gtTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGa > 1:1589157/1‑43 (MQ=255) gtTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGa > 1:2883792/1‑43 (MQ=255) cggctcgCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTg < 1:2247140/49‑1 (MQ=255) ggctcgCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCa < 1:2022500/71‑1 (MQ=255) ctcgCAGGAGCGGGCTCACCAGAACGCGTTCGATTTCCACttttt < 1:1937919/45‑1 (MQ=255) tcgCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCttt < 1:1757526/51‑1 (MQ=255) gCAGGAACGGGCTCACCAGAACGCGTTCGATTTGCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCga < 1:3449388/71‑1 (MQ=255) gCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGc < 1:3576664/53‑1 (MQ=255) gCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGc < 1:244717/53‑1 (MQ=255) aGGAGCGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGc > 1:4325581/1‑67 (MQ=255) aGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCt < 1:3652245/45‑1 (MQ=255) aGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGc > 1:3582332/1‑67 (MQ=255) aGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCgata < 1:1976507/71‑3 (MQ=255) aaCGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCAt > 1:2017486/1‑59 (MQ=255) | ACTTCTGCACTGGAAGGCAGGTTCAGACAATCCCCTACTTCTTCCAGTGTTTGCTCGGCTCGCAGGAACGGGCTCACCAGAACGCGTTCGATTTCCACTTTTTGACCTTTCAGCCAGTTCGCCATCAGGCGAGA > W3110S.gb/2462035‑2462168 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |