| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,771,105 | G→A | 11.5% | N79N (AAC→AAT) | ypjL ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,771,105 | 0 | G | A | 11.5% | 227.5 / 16.9 | 79 | N79N (AAC→AAT) | ypjL | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (33/36); new base A (5/4); total (39/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.48e-01 | |||||||||||
CTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCTTGT > W3110S.gb/2771036‑2771172 | ctctTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGt < 1:4002396/71‑1 (MQ=255) tctTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGtt > 1:3593592/1‑71 (MQ=255) ctTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGtt > 1:2587377/1‑70 (MQ=255) tttGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTg < 1:2833688/67‑1 (MQ=255) tttGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTtca > 1:2168328/1‑71 (MQ=255) ttGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTtcat > 1:3186349/1‑71 (MQ=255) tGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTtc < 1:1982758/68‑1 (MQ=255) acgacgCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCAc < 1:874039/71‑1 (MQ=255) acgCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACtga < 1:355920/71‑1 (MQ=255) cgCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACtgat > 1:2230304/1‑71 (MQ=255) gCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACtgatg < 1:2858130/71‑1 (MQ=255) gCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACtgat > 1:1732626/1‑70 (MQ=255) ccAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACtgat > 1:877228/1‑69 (MQ=255) ccAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACtgat > 1:1019030/1‑69 (MQ=255) ccAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACtg < 1:1995833/67‑1 (MQ=255) cAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATgag < 1:2832436/71‑1 (MQ=255) cAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATgag < 1:2607104/71‑1 (MQ=255) aGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTTATgaga < 1:2983871/71‑1 (MQ=255) gTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATgagag < 1:556129/71‑1 (MQ=255) tCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGt < 1:254249/54‑1 (MQ=255) gCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGaaa > 1:756191/1‑71 (MQ=255) ccGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAAt < 1:4090573/71‑1 (MQ=255) ccGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAAt > 1:1900548/1‑71 (MQ=255) cGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTATTCATCACTGATGAGAGAAATg > 1:4247226/1‑71 (MQ=255) gTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACt < 1:3801876/57‑1 (MQ=255) tACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGt > 1:1886239/1‑48 (MQ=255) cTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATgagaga < 1:670960/63‑1 (MQ=255) cTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGt < 1:3610936/71‑1 (MQ=255) cTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTATTCATCACTGATGAGAGAAATGCCg > 1:1527386/1‑70 (MQ=255) cccGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTc > 1:2789991/1‑70 (MQ=255) ccGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTtca > 1:1175161/1‑46 (MQ=255) ccGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGaa > 1:3764774/1‑61 (MQ=255) ccGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTc < 1:4564597/69‑1 (MQ=255) gTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTtca < 1:2613098/44‑1 (MQ=255) tCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTg > 1:2723829/1‑39 (MQ=255) cAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTtcatc > 1:427385/1‑44 (MQ=255) aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTtcat > 1:4592754/1‑42 (MQ=255) aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTtcat > 1:4383062/1‑42 (MQ=255) aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTtcat > 1:4597855/1‑42 (MQ=255) aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACtgatga > 1:401205/1‑51 (MQ=255) aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGc < 1:2321843/71‑1 (MQ=255) aaTCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGc > 1:3838383/1‑71 (MQ=255) aTCGACAGCGCACCAATCAGCACCGTCGGCATATTATTCATCACTGATGAGAGAAATGCCGTCAAGAAGcc < 1:4481008/71‑1 (MQ=255) tCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATgaga < 1:456215/51‑1 (MQ=255) tCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATg < 1:2142033/57‑1 (MQ=255) tCGACAGCGCACCAATCAGCACCGTCGGAATATTGTTCATCACtgatg > 1:277467/1‑48 (MQ=255) acGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACtgatg > 1:2624588/2‑48 (MQ=255) cGACAGCGCACCAATCATCACCGTCGGCATATTCTtcatga > 1:1790366/1‑39 (MQ=21) gACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGt > 1:3439728/1‑71 (MQ=255) gACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGt > 1:3440329/1‑71 (MQ=255) cAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGc > 1:1660854/1‑54 (MQ=255) aGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGaa > 1:3795644/1‑49 (MQ=255) gcgcACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCg < 1:2201650/71‑1 (MQ=255) aCCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATg < 1:2260809/47‑1 (MQ=255) aCCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCaaga < 1:1131495/56‑1 (MQ=255) aCCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACg < 1:251628/71‑1 (MQ=255) aCCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACg < 1:2795452/71‑1 (MQ=255) aCCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGAGCCGAACg < 1:3356136/71‑1 (MQ=255) cAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGt < 1:4452322/49‑1 (MQ=255) aTCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTca > 1:2816371/1‑69 (MQ=255) tCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCaa < 1:4493800/49‑1 (MQ=255) tCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCt < 1:2395710/71‑1 (MQ=255) cAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTg > 1:4016876/1‑71 (MQ=255) aGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGc > 1:2251193/1‑71 (MQ=255) aGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGc > 1:3783117/1‑71 (MQ=255) aGCACCGTCGGCATATTATTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGc > 1:369185/1‑71 (MQ=255) aGCACCGTCGGCATATTATTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGc > 1:374664/1‑71 (MQ=255) aCCGTCGGCATATTATTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCa > 1:4602089/1‑71 (MQ=255) cGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCATa < 1:1044727/71‑1 (MQ=255) cGTCGGCATATTATTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCATa < 1:3795593/71‑1 (MQ=255) cGTCGGCATATTATTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCATa < 1:3790198/71‑1 (MQ=255) gTCGGCATATTATTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCATaa < 1:3941305/71‑1 (MQ=255) ggCATATTGTTAATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTc > 1:4392519/1‑57 (MQ=255) gCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCATAAccc < 1:1348416/70‑1 (MQ=255) cATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGc < 1:322189/41‑1 (MQ=255) tatTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGcc < 1:608587/40‑1 (MQ=255) tatTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGcc < 1:714404/40‑1 (MQ=255) tatTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTc < 1:1426359/53‑1 (MQ=255) atTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCTTGt > 1:1892031/1‑71 (MQ=255) ttGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCa > 1:1723478/1‑59 (MQ=255) ttGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCTTGt < 1:2875266/70‑1 (MQ=255) | CTCTTTGACGACGCCAGTCGCCGTACTCCCGTCAATCGACAGCGCACCAATCAGCACCGTCGGCATATTGTTCATCACTGATGAGAGAAATGCCGTCAAGAAGCCGGTGCCGAACGTCGCTGCCCATAACCCCTTGT > W3110S.gb/2771036‑2771172 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |