| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,966,215 | T→G | 17.6% | K292N (AAA→AAC) | ptsP ← | fused PEP‑protein phosphotransferase (enzyme I) of PTS system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,966,215 | 0 | T | G | 17.6% | 92.7 / 29.8 | 57 | K292N (AAA→AAC) | ptsP | fused PEP‑protein phosphotransferase (enzyme I) of PTS system |
| Reads supporting (aligned to +/- strand): ref base T (13/34); new base G (3/7); total (16/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCC > W3110S.gb/2966147‑2966281 | ccTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCttt < 1:2545790/71‑1 (MQ=255)ccTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCttt < 1:2364255/71‑1 (MQ=255) ttGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCttt > 1:2195826/1‑69 (MQ=255) aTTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTgaga > 1:734809/1‑70 (MQ=255) aGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATatt > 1:3640474/1‑69 (MQ=255) gCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGt < 1:1214890/70‑1 (MQ=255) gTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTAt < 1:2957532/71‑1 (MQ=255) gTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTAt < 1:262105/71‑1 (MQ=255) gTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTAt < 1:1392910/71‑1 (MQ=255) atcaAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCa < 1:896171/70‑1 (MQ=255) atcaAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCAc > 1:3989988/1‑71 (MQ=255) atcaAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCAc > 1:3406014/1‑71 (MQ=255) tcaAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACt < 1:2959383/71‑1 (MQ=255) aaGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTga < 1:3857874/56‑1 (MQ=255) aaGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACt > 1:4117336/1‑69 (MQ=255) aaGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACt > 1:2343318/1‑69 (MQ=255) aGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAg < 1:1580779/71‑1 (MQ=255) gATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAgc < 1:4169272/71‑1 (MQ=255) aTGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAgcg < 1:271226/71‑1 (MQ=255) aaaCAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAgcgc < 1:1883022/69‑1 (MQ=255) aaCAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCttt < 1:1424711/47‑1 (MQ=255) aaCAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCttt < 1:1305468/47‑1 (MQ=255) aaCAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCa > 1:3929001/1‑60 (MQ=255) cAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCa > 1:4369707/1‑70 (MQ=255) aGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACt < 1:3870948/59‑1 (MQ=255) aGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCa < 1:4133556/69‑1 (MQ=255) gCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAAc < 1:2516426/71‑1 (MQ=255) cAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAgcg > 1:4105980/1‑62 (MQ=255) cAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAAc > 1:3532856/1‑70 (MQ=255) cAATCGCTGACCCAGCGCAAGTAAATCGCCAGCCCGCTCTTTGAGATAGTTAT‑ACTTAGCGCGGCAAACTg < 1:2504195/71‑1 (MQ=255) aaTCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTg < 1:517807/71‑1 (MQ=255) aTCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGt < 1:1993432/71‑1 (MQ=255) tCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCa < 1:955225/64‑1 (MQ=255) tCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGtt < 1:4435283/71‑1 (MQ=255) tCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGt < 1:4472393/70‑1 (MQ=255) gCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCg < 1:4116624/71‑1 (MQ=255) cTGACCCAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATc < 1:2420473/48‑1 (MQ=255) aCCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCg > 1:3981269/1‑67 (MQ=255) aCCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCaa < 1:2604348/71‑1 (MQ=255) aCCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCa > 1:976517/1‑70 (MQ=255) cccAGCGCACGTAAATCGCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCaaa < 1:3969866/71‑1 (MQ=255) ccAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCa < 1:3909372/44‑1 (MQ=255) cAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAAtt < 1:1980837/71‑1 (MQ=255) aGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAAttt > 1:1590963/1‑71 (MQ=255) aGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAAttt > 1:1585709/1‑71 (MQ=255) aGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAAttt < 1:1996102/71‑1 (MQ=255) gcgcACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTg < 1:3421364/58‑1 (MQ=255) cgcACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGtt > 1:120580/1‑59 (MQ=255) gcACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTc < 1:4340214/71‑1 (MQ=255) cGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAAt < 1:1249127/71‑1 (MQ=255) gTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACt < 1:2450207/37‑1 (MQ=255) gTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATg < 1:1181109/71‑1 (MQ=255) tAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGa < 1:2455823/71‑1 (MQ=255) aaTCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCGAATTTTTCAATGAcc < 1:1742786/71‑1 (MQ=255) aaTCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCa < 1:1600479/65‑1 (MQ=255) tCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCaa < 1:789908/64‑1 (MQ=255) tCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGTCCGt < 1:2702831/71‑1 (MQ=255) gCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGtt < 1:1709456/70‑1 (MQ=255) gCCAGCCCGCTCTTTGACATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGttt > 1:3765006/1‑71 (MQ=255) gCCAGCCCGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGttt < 1:1425888/71‑1 (MQ=255) cAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGtt < 1:1061405/45‑1 (MQ=255) cGCTCGTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGtttt < 1:285844/65‑1 (MQ=255) ctctTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGcc < 1:2542343/70‑1 (MQ=255) tctTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGc > 1:2146050/1‑68 (MQ=255) | CCTTGATTAGCGTCATCAAGATGAAACAGCAATCGCTGACCCAGCGCACGTAAATCGCCAGCCCGCTCTTTGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCC > W3110S.gb/2966147‑2966281 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |