| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,113,649 | C→T | 16.7% | A1374T (GCA→ACA) | yghJ ← | predicted inner membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,113,649 | 0 | C | T | 16.7% | 183.1 / 25.3 | 72 | A1374T (GCA→ACA) | yghJ | predicted inner membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base C (35/25); new base T (7/5); total (42/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.51e-01 | |||||||||||
GCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGGTTGTTGCTCTCCTCCAGATATT > W3110S.gb/3113580‑3113717 | gCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCACATTCCTTCAGCTGTGCg > 1:2905597/1‑71 (MQ=255) cAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCg < 1:3375777/70‑1 (MQ=255) agagGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTa > 1:1498157/1‑71 (MQ=255) aggagTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACa > 1:2047920/1‑71 (MQ=255) aggagTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACa < 1:1954324/71‑1 (MQ=255) gTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATcag > 1:1191940/1‑71 (MQ=255) gTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATcag > 1:1445332/1‑71 (MQ=255) ccATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGc > 1:4614842/1‑59 (MQ=255) ccATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATcag > 1:997802/1‑68 (MQ=255) cTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGTGTACATCAGCAGACgg > 1:3695515/1‑71 (MQ=255) cTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGTGTACATCAGCAGACgg > 1:1506836/1‑71 (MQ=255) cTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGa > 1:2230484/1‑68 (MQ=255) cTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACgg > 1:3988970/1‑71 (MQ=255) tGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCTTACATCAGCAGACg > 1:4502752/1‑69 (MQ=255) tGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACAt > 1:617664/1‑60 (MQ=255) tGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGt > 1:3251542/1‑71 (MQ=255) gATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGTTTACa < 1:1821009/57‑1 (MQ=255) gATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACg < 1:1307161/67‑1 (MQ=255) gATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTc < 1:880244/70‑1 (MQ=255) gATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTc < 1:393875/70‑1 (MQ=255) tACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCAcc > 1:1721951/1‑71 (MQ=255) cATTTCTTGATATCAAAGTTTTTATCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCg > 1:2665345/1‑70 (MQ=255) tttCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGt < 1:3390723/47‑1 (MQ=255) tttCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCAc > 1:3737035/1‑71 (MQ=255) tttCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCAc < 1:1272549/71‑1 (MQ=255) tttCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCAc > 1:2426643/1‑71 (MQ=255) tCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCAc > 1:980255/1‑69 (MQ=255) cTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCg > 1:409542/1‑43 (MQ=255) ttGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCAccgcc > 1:626066/1‑71 (MQ=255) atatCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATcagc > 1:95295/1‑48 (MQ=255) atCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTg > 1:241434/1‑71 (MQ=255) tCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGc < 1:4354575/71‑1 (MQ=255) aaaGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGa > 1:1177266/1‑46 (MQ=255) aaaGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGcc < 1:4254296/70‑1 (MQ=255) aaaGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGcc < 1:985252/70‑1 (MQ=255) aGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGt < 1:1631508/48‑1 (MQ=255) aGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCAt > 1:1834696/1‑71 (MQ=255) aGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCAt > 1:3487938/1‑71 (MQ=255) gTTTTTCTCTGCCCATTCCTTCAGCTGTGTGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATg > 1:4227742/1‑71 (MQ=255) gTTTTTCTCTGCCCATTCCTTCAGCTGTGTGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATg > 1:4277535/1‑71 (MQ=255) gTTTTTCTCTGCCCATTCCTTCAGCTGTGTCTACAt < 1:296354/36‑1 (MQ=37) gTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATg > 1:4359195/1‑71 (MQ=255) tttttCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGAc < 1:1437238/43‑1 (MQ=255) tttttCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGAc < 1:1837709/43‑1 (MQ=255) ttttCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGt > 1:2318672/1‑62 (MQ=255) tttCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACgg > 1:4567437/1‑43 (MQ=255) tttCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGcc < 1:3647795/70‑1 (MQ=255) tttCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCt > 1:2907438/1‑71 (MQ=255) ttCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGTAGACGGTCACCCGCACCGCCGCGTGCCCATGCCt < 1:413973/70‑1 (MQ=255) ttCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGcc < 1:1250667/69‑1 (MQ=255) tctcTGCCCATTCCTTCAGCTGTGTGTACATCAGCAGACGGTCAccc > 1:3008595/1‑47 (MQ=255) tctcTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTgg > 1:3546061/1‑71 (MQ=255) ctctGCCCATTCCTTCAGCTGTGTGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGc < 1:1252056/66‑1 (MQ=255) ctctGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCt < 1:4560170/68‑1 (MQ=255) ctctGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTgg < 1:2379456/70‑1 (MQ=255) tGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACcgccgc > 1:842242/1‑53 (MQ=255) tGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGcc > 1:1031107/1‑58 (MQ=255) tGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGgttgt > 1:668957/1‑71 (MQ=255) tGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGgtt < 1:279003/69‑1 (MQ=255) cccATTCCTTCAGCTGTGTGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCAAGCCTGGttgttg > 1:1227662/1‑71 (MQ=255) cccATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGgttgttc > 1:2642793/1‑70 (MQ=255) cccATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGgttgt < 1:3896960/69‑1 (MQ=255) ccATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGGTTGTTGc > 1:4279999/1‑71 (MQ=255) ccATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGGTTGTTGc < 1:882298/71‑1 (MQ=255) cATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGgttgt > 1:4379268/1‑67 (MQ=255) ttCCTTCAGCTGTGTGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGgttgt < 1:1621546/65‑1 (MQ=255) cTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGGTTGTTGctct > 1:1347184/1‑68 (MQ=255) tCAGCTGTGTGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGGTTGTTGCTctcctc > 1:1562415/1‑70 (MQ=255) gCTGTGTGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGGTTGTTGCTCTCCTCCa < 1:2714523/69‑1 (MQ=255) cTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGGTTGTTGCTCTCCTCCAGa < 1:1191309/70‑1 (MQ=255) gtgCGTACATCAGCAGACGGTCACCCGAACCGCCGCGTGCCCATGCCt < 1:3651261/48‑1 (MQ=255) tgCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGGTTGTTGCTCTCCTCCAGatat < 1:1084988/70‑1 (MQ=255) tgCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGGTTGTTGCTCTCCTCCAGATAtt < 1:1867550/71‑1 (MQ=255) cGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGgttgt > 1:239964/1‑51 (MQ=255) | GCAGAGGAGTGCCATCTGGATACCATTTCTTGATATCAAAGTTTTTCTCTGCCCATTCCTTCAGCTGTGCGTACATCAGCAGACGGTCACCCGCACCGCCGCGTGCCCATGCCTGGTTGTTGCTCTCCTCCAGATATT > W3110S.gb/3113580‑3113717 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |