| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,191,865 | G→A | 7.5% | A116T (GCC→ACC) | yqiK → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,191,865 | 0 | G | A | 7.5% | 168.9 / 3.5 | 53 | A116T (GCC→ACC) | yqiK | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (30/19); new base A (2/2); total (32/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.95e-01 | |||||||||||
GATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGT > W3110S.gb/3191805‑3191932 | gATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCt < 1:346705/71‑1 (MQ=255) aTGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTg > 1:318873/1‑60 (MQ=255) gtagtaGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTACCCAGACGCTggg > 1:3885540/1‑71 (MQ=255) gtagtCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTg < 1:1459574/66‑1 (MQ=255) tagtCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCaa > 1:634368/1‑71 (MQ=255) gtCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACg > 1:3958238/1‑71 (MQ=255) gCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCa < 1:2645053/70‑1 (MQ=255) gCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCAc > 1:2822749/1‑71 (MQ=255) ctttctttGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCAcc < 1:3819344/71‑1 (MQ=255) ctttctttGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCAcc < 1:406874/71‑1 (MQ=255) ctttctttGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTACCCAGACGCTGGGGCAACGCAcc > 1:2366694/1‑71 (MQ=255) tttctttGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCAccc > 1:3372799/1‑71 (MQ=255) tgtgCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGc > 1:1561693/1‑71 (MQ=255) tgCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTg < 1:160352/65‑1 (MQ=255) tgCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGcc < 1:1625306/70‑1 (MQ=255) cggTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTTGGGCAACGCACCCTGTCGCCTg > 1:554390/2‑69 (MQ=255) gTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGa < 1:2688753/71‑1 (MQ=255) aaaCCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTg < 1:3058017/57‑1 (MQ=255) aaaCCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGa > 1:3433628/1‑68 (MQ=255) aaaCCTTCAGTAGAAGGGATTGCCACCGCTACCCAGACGCTGGGGCAACGCACCCt < 1:1852785/56‑1 (MQ=255) aaaCCTTCAGTAGAAGGGATTGCAACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACt > 1:279934/1‑70 (MQ=255) aCCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTAc > 1:1835738/1‑71 (MQ=255) ccTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACg > 1:3671119/1‑71 (MQ=255) tCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGcc > 1:559314/1‑56 (MQ=255) tCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTAt > 1:178825/1‑71 (MQ=255) aGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATgt < 1:331341/71‑1 (MQ=255) tAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATgttg < 1:2728816/71‑1 (MQ=255) gAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCa < 1:2975207/40‑1 (MQ=255) gAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGc > 1:3239210/1‑49 (MQ=255) gAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATgttggt > 1:4590199/1‑71 (MQ=255) gAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATgttgg < 1:3235043/70‑1 (MQ=255) aaGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATgttggtt > 1:1863504/1‑71 (MQ=255) aGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATgttggtt > 1:1734049/1‑70 (MQ=255) aGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATgttggtt > 1:1613444/1‑70 (MQ=255) gggATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATgttggttg > 1:1512944/1‑70 (MQ=255) gggATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATgttggttg > 1:2854374/1‑70 (MQ=255) ttGCCACCGCTGCCCAGCCGCTGGGGCAACGCACCCTGTCGcc > 1:3855892/1‑43 (MQ=255) ttGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGa > 1:2199982/1‑70 (MQ=255) gCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGa > 1:3481855/1‑47 (MQ=255) gCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATa > 1:2881908/1‑70 (MQ=255) ccACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTTAAGACTTACGTATGTTGGTTGAAGATaa < 1:1402185/70‑1 (MQ=255) ccACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATaaa < 1:2341379/71‑1 (MQ=255) ccACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATaaa < 1:3994330/71‑1 (MQ=255) cACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAAt > 1:2782248/1‑71 (MQ=255) aCCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTa > 1:3193218/1‑52 (MQ=255) aCCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAAtt > 1:3522402/1‑71 (MQ=255) ccGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAAttt < 1:4459772/71‑1 (MQ=255) ccGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAAttt < 1:468527/71‑1 (MQ=255) ccGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCATGAAGACTTACGTATGTTGGTTGAAGATAAAttt > 1:3668266/1‑71 (MQ=255) ccGCTACCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAAttt < 1:927860/71‑1 (MQ=255) cGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTg > 1:3817169/1‑71 (MQ=255) gCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGt > 1:534540/1‑71 (MQ=255) gCTGACCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGt > 1:3528166/1‑71 (MQ=255) cTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGt < 1:3267259/47‑1 (MQ=255) gCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGAt < 1:3343082/60‑1 (MQ=255) | GATGTAGTAGTCGCTTTCTTTGTGCGGGTAAAACCTTCAGTAGAAGGGATTGCCACCGCTGCCCAGACGCTGGGGCAACGCACCCTGTCGCCTGAAGACTTACGTATGTTGGTTGAAGATAAATTTGT > W3110S.gb/3191805‑3191932 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |