Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,244,017 G→A 9.7% Q86Q (CAG→CAA)  exuT → hexuronate transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,244,0170GA 9.7% 287.2 / 16.0 93Q86Q (CAG→CAA) exuThexuronate transporter
Reads supporting (aligned to +/- strand):  ref base G (47/37);  new base A (6/3);  total (53/40)
Fisher's exact test for biased strand distribution p-value = 7.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.63e-01

ACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTA  >  W3110S.gb/3243947‑3244083
                                                                      |                                                                  
aCCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAg                                                                    >  1:1510329/1‑71 (MQ=255)
  cTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTa                                                                  <  1:190289/71‑1 (MQ=255)
    gACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTAtt                                                                >  1:2182533/1‑71 (MQ=255)
    gACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTAt                                                                 >  1:3929040/1‑70 (MQ=255)
    gACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTAt                                                                 >  1:2267731/1‑70 (MQ=255)
      cgcgTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAATATTcc                                                              >  1:1384097/1‑71 (MQ=255)
      cgcgTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAATATTcc                                                              >  1:3299404/1‑71 (MQ=255)
       gcgTAACACTGTGGCGGCAGCTGCGCCCACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTcc                                                              >  1:1558590/1‑70 (MQ=255)
       gcgTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTAt                                                                 <  1:3498315/67‑1 (MQ=255)
        cgTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCt                                                             <  1:2897286/70‑1 (MQ=255)
         gTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCta                                                            >  1:1159015/1‑70 (MQ=255)
            acacTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTatat                                                         >  1:1765036/1‑70 (MQ=255)
            acacTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTatat                                                         >  1:1953122/1‑70 (MQ=255)
             cacTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATatca                                                       <  1:8382/71‑1 (MQ=255)
             cacTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATatca                                                       <  1:330893/71‑1 (MQ=255)
                tgtgGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCg                                                    >  1:3386397/1‑71 (MQ=255)
                  tgGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAATATTCCTATATCATCGc                                                   <  1:4176175/70‑1 (MQ=255)
                    gcggcAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAg                                                 <  1:983918/70‑1 (MQ=255)
                     cggcAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAg                                                 >  1:4529237/1‑69 (MQ=255)
                     cggcAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGc                                                <  1:1797487/70‑1 (MQ=255)
                       gcAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTa                                             <  1:3648174/71‑1 (MQ=255)
                         aGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTAtt                                           >  1:549410/1‑71 (MQ=255)
                          gCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTc                                          <  1:2578318/71‑1 (MQ=255)
                           cTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATatc                                                        <  1:498826/56‑1 (MQ=255)
                              cgcCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTctgct                                      <  1:1772660/71‑1 (MQ=255)
                              cgcCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTctgct                                      >  1:1554730/1‑71 (MQ=255)
                               gcCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTctgctg                                     >  1:3679331/1‑71 (MQ=255)
                                ccAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCtgctgc                                    <  1:748722/71‑1 (MQ=255)
                                ccAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATCCtgctgc                                    >  1:1699423/1‑71 (MQ=255)
                                 cAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATatcat                                                      >  1:815844/1‑52 (MQ=255)
                                 cAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTgctgct                                   >  1:3558234/1‑71 (MQ=255)
                                 cAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTgctgct                                   >  1:4578149/1‑71 (MQ=255)
                                 cAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTgctgct                                   >  1:2767791/1‑71 (MQ=255)
                                  aaCTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTgctgct                                   >  1:1713374/1‑70 (MQ=255)
                                   aCTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTta                                 <  1:3354395/71‑1 (MQ=255)
                                    ctctGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTtat                                <  1:2184373/71‑1 (MQ=255)
                                     tctGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTtata                               >  1:465283/1‑71 (MQ=255)
                                      ctGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATAc                              >  1:4469410/1‑71 (MQ=255)
                                       tGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTtata                               <  1:1988414/69‑1 (MQ=255)
                                       tGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATAc                              >  1:1442434/1‑70 (MQ=255)
                                       tGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACg                             >  1:115077/1‑71 (MQ=255)
                                        gATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACgg                            >  1:2730187/1‑71 (MQ=255)
                                         aTGGAAGAGTTAAACATCTCCACCCAACAGTATTCCta                                                            >  1:4069065/1‑38 (MQ=255)
                                         aTGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCa                                                  <  1:4546062/48‑1 (MQ=255)
                                         aTGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACgg                            <  1:837087/70‑1 (MQ=255)
                                          tGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTgctgct                                   <  1:3437051/62‑1 (MQ=255)
                                          tGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGt                           >  1:3971664/1‑70 (MQ=255)
                                          tGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTc                          <  1:2905006/71‑1 (MQ=255)
                                          tGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTc                          <  1:2389897/71‑1 (MQ=255)
                                          tGGAAGAGTTAAACATCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGt                           >  1:185352/1‑70 (MQ=255)
                                          tGGAAGAGTTAAACATCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTc                          <  1:3955196/71‑1 (MQ=255)
                                           ggAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTctg                                        <  1:2868956/56‑1 (MQ=255)
                                           ggAAGAGTTAAACATCTCCACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTta                                 >  1:2904965/1‑63 (MQ=255)
                                            gAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCAt                        <  1:489451/71‑1 (MQ=255)
                                            gAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCAt                        >  1:2524276/1‑71 (MQ=255)
                                             aaGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTctgct                                      >  1:2682499/1‑56 (MQ=255)
                                              agagTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGc                      <  1:2536592/71‑1 (MQ=255)
                                              agagTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCCGCTTATACGGTCATGc                      <  1:1990455/71‑1 (MQ=255)
                                              agagTTAAACATCTACACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATg                       >  1:3438182/1‑70 (MQ=255)
                                               gagTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGc                                                >  1:4370456/1‑44 (MQ=255)
                                               gagTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATAc                              >  1:2024525/1‑62 (MQ=255)
                                               gagTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATAc                              >  1:1925774/1‑62 (MQ=255)
                                               gagTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACTGTCATGCa                     <  1:1618197/71‑1 (MQ=255)
                                                  ttAAACATCTCCACCCAACAGTATTCCTATATCATCGc                                                   <  1:4443507/38‑1 (MQ=255)
                                                  ttAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTctg                                        <  1:4263/49‑1 (MQ=255)
                                                  ttAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACgg                            <  1:333241/61‑1 (MQ=255)
                                                  ttAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACgg                            <  1:4033590/61‑1 (MQ=255)
                                                  ttAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCAt                        <  1:3432789/65‑1 (MQ=255)
                                                  ttAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAAcc                  <  1:2761103/71‑1 (MQ=255)
                                                    aaaCATCTCCACCCAACAGTATTCCTATATCATCGCa                                                  >  1:697844/1‑37 (MQ=255)
                                                    aaaCATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTa                                             <  1:2083264/42‑1 (MQ=255)
                                                    aaaCATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACg                             >  1:1733541/1‑58 (MQ=255)
                                                    aaaCATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCgg                >  1:1657388/1‑71 (MQ=255)
                                                    aaaCATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCgg                >  1:1983414/1‑71 (MQ=255)
                                                    aaaCATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCgg                >  1:254069/1‑71 (MQ=255)
                                                    aaaCATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCgg                >  1:2612723/1‑71 (MQ=255)
                                                      aCATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCgg                >  1:3234187/1‑69 (MQ=255)
                                                      aCATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCgg                >  1:2116465/1‑69 (MQ=255)
                                                      aCATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGt               <  1:1084478/70‑1 (MQ=255)
                                                        aTCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCaa                    <  1:457215/63‑1 (MQ=255)
                                                        aTCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCgg                >  1:4206573/1‑67 (MQ=255)
                                                        aTCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGc            <  1:2566494/71‑1 (MQ=255)
                                                        aTCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACAGGTa              >  1:2978696/1‑69 (MQ=255)
                                                         tctcCACCCAACAGTATTCCTATATCATCGCAGCCTATTctgct                                      >  1:1731284/1‑44 (MQ=255)
                                                           tcCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAg             >  1:2435735/1‑67 (MQ=255)
                                                             cACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGt        >  1:2861598/1‑70 (MQ=255)
                                                             cACCCAACAATATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTc                          <  1:1564177/52‑1 (MQ=255)
                                                              aCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTa      <  1:4409861/71‑1 (MQ=255)
                                                              aCCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTa      >  1:2925107/1‑71 (MQ=255)
                                                                ccAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCtt                                  >  1:3586831/1‑41 (MQ=255)
                                                                  aaCAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTa  >  1:2590367/1‑71 (MQ=255)
                                                                  aaCAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTa  >  1:4238494/1‑71 (MQ=255)
                                                                  aaCAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTa  <  1:313024/71‑1 (MQ=255)
                                                                     aGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGtt       >  1:171354/1‑63 (MQ=255)
                                                                      |                                                                  
ACCTGACGCGTAACACTGTGGCGGCAGCTGCGCCAACTCTGATGGAAGAGTTAAACATCTCCACCCAACAGTATTCCTATATCATCGCAGCCTATTCTGCTGCTTATACGGTCATGCAACCGGTAGCAGGTTATGTA  >  W3110S.gb/3243947‑3244083

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑