| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,253,271 | C→T | 11.9% | V99V (GTC→GTT) | yhaK → | predicted pirin‑related protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,253,271 | 0 | C | T | 11.9% | 196.7 / 13.6 | 67 | V99V (GTC→GTT) | yhaK | predicted pirin‑related protein |
| Reads supporting (aligned to +/- strand): ref base C (31/28); new base T (3/5); total (34/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGATAGCGAAGGCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTG > W3110S.gb/3253201‑3253339 | gcgATAGCGAAGGCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTc < 1:1717118/71‑1 (MQ=255) aTAGCGAAGGCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGc < 1:1288676/71‑1 (MQ=255) tAGCGAAGGCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCT‑CTCTCTACCCAGCCGGGTGTCAGCta < 1:943390/71‑1 (MQ=255) aGCGAAGGCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCta > 1:2535107/1‑71 (MQ=255) aaTCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTTAGCTATAGCGAaca < 1:2734301/71‑1 (MQ=255) tCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAacac > 1:3365075/1‑70 (MQ=255) tCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCGCTCTACCCAGCCGGGTGTCAGCtat < 1:3114425/61‑1 (MQ=255) cATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCCGCCGGGTGTCAGCTATAGCGAaca > 1:2379753/1‑68 (MQ=255) cATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACaa > 1:4581859/1‑71 (MQ=255) gTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGaa > 1:3407982/1‑63 (MQ=255) ttCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCta < 1:582021/55‑1 (MQ=255) ttCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAAt < 1:58434/68‑1 (MQ=255) ggCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCa < 1:3727493/71‑1 (MQ=255) cAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTTAGCTATAGCGAACACAATCTCAGCa > 1:1437506/1‑68 (MQ=255) cAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGa < 1:491405/54‑1 (MQ=255) cAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAAtc > 1:1424981/1‑62 (MQ=255) cAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCa > 1:1459730/1‑68 (MQ=255) cgcCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGAc > 1:1059104/1‑70 (MQ=255) ccGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAg < 1:2248705/61‑1 (MQ=255) ccGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCaaa > 1:940250/1‑65 (MQ=255) ccGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACaa > 1:1075355/1‑70 (MQ=255) ggAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAAtc < 1:2702932/55‑1 (MQ=255) gagaGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCg > 1:189723/1‑71 (MQ=255) agagGCGTTGCTGCTCTCTACCCAGCCGGGTGTTAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGt < 1:2957907/71‑1 (MQ=255) agagGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGt < 1:1723871/71‑1 (MQ=255) gagGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAAc > 1:3939593/1‑67 (MQ=255) agGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGtt > 1:478677/1‑70 (MQ=255) ggCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTaa > 1:4330547/1‑71 (MQ=255) gCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAAc < 1:2962463/71‑1 (MQ=255) gCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAAc < 1:1718668/71‑1 (MQ=255) gTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCg < 1:1629326/64‑1 (MQ=255) gTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAAcgc > 1:3732434/1‑71 (MQ=255) ttGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAg < 1:3106138/55‑1 (MQ=255) ttGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAAcgcg < 1:3606393/71‑1 (MQ=255) tgctgcTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAAcgcg > 1:1498027/1‑70 (MQ=255) tgctgcTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAAcgcg > 1:2122902/1‑70 (MQ=255) gctgctCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGaa > 1:387458/1‑71 (MQ=255) tgctCTCTACCCAGCCGGGTGTTAGCTATAGCGAACACAATCTCAGCa < 1:912946/48‑1 (MQ=255) tgctCTCTACCCAGCCGGGTGTTAGCTATAGCGAACACAATCTCAGCa < 1:919942/48‑1 (MQ=255) tgctCTCTACCCAGCCGGGTGTTAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATg > 1:1001638/1‑71 (MQ=255) tgctCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAAcgcg < 1:2506448/67‑1 (MQ=255) tgctCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGa < 1:2485344/68‑1 (MQ=255) gctCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCg < 1:534522/58‑1 (MQ=255) gctCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATg > 1:3847560/1‑70 (MQ=255) ctctctACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGtt < 1:1333984/59‑1 (MQ=255) ctctctACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGc < 1:4196354/70‑1 (MQ=255) tctctACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCa > 1:1009158/1‑70 (MQ=255) ctctACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGc > 1:2224858/1‑71 (MQ=255) tctACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGAc > 1:4559483/1‑48 (MQ=255) tctACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGtt < 1:2419866/56‑1 (MQ=255) tctACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAAc < 1:3951560/59‑1 (MQ=255) tctACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCt > 1:4599051/1‑71 (MQ=255) tctACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCt > 1:3844977/1‑71 (MQ=255) tACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGtt < 1:3092556/54‑1 (MQ=255) aCCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCttt < 1:2197872/70‑1 (MQ=255) cccAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTgg > 1:3283331/1‑71 (MQ=255) cccAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTgg > 1:2978336/1‑71 (MQ=255) cccAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTg < 1:4612296/70‑1 (MQ=255) ccAGCCGGGTGTTAGCTATAGCGAACACAATCTCAGCAAAGACaa > 1:4505755/1‑45 (MQ=255) cAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCt < 1:4557488/65‑1 (MQ=255) aGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTg > 1:993079/1‑71 (MQ=255) aGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTg > 1:989643/1‑71 (MQ=255) aGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTg < 1:4543018/71‑1 (MQ=255) gggTGTTAGCTATAGCGAACACAATCTCAGCAAAGACaa < 1:4542399/39‑1 (MQ=255) ggTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGc > 1:793377/1‑71 (MQ=255) ggTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGc < 1:3640583/71‑1 (MQ=255) tgtCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAAt > 1:2722603/1‑51 (MQ=255) gtCAGCGATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTg > 1:592733/1‑71 (MQ=255) | GCGATAGCGAAGGCAATCATGTTCAGGCCAGCGCCGGAGAGGCGTTGCTGCTCTCTACCCAGCCGGGTGTCAGCTATAGCGAACACAATCTCAGCAAAGACAAACCGTTAACGCGAATGCAGCTTTGGCTGGACGCCTG > W3110S.gb/3253201‑3253339 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |