Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 4,371,273 Δ2 bp 100% intergenic (‑6/+299) dcuA ← / ← aspA C4‑dicarboxylate antiporter/aspartate ammonia‑lyase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,371,2730A.95.0% 69.9 / ‑1.2 20intergenic (‑6/+300)dcuA/aspAC4‑dicarboxylate antiporter/aspartate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base . (9/10);  total (9/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.
*W3110S.gb4,371,2740A.95.0% 68.6 / ‑1.5 20intergenic (‑7/+299)dcuA/aspAC4‑dicarboxylate antiporter/aspartate ammonia‑lyase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base . (9/10);  minor base T (0/1);  total (9/11)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTT  >  W3110S.gb/4371204‑4371334
                                                                     ||                                                            
ggTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGaa                                                              <  1:1330283/71‑1 (MQ=255)
            gcagcTTCTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATa                                                <  1:197859/71‑1 (MQ=255)
                cTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCg                                            <  1:1126137/71‑1 (MQ=255)
                    tctgtctgATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCAt                                        >  1:315381/1‑71 (MQ=255)
                          tgatgaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAAt                                  >  1:736733/1‑71 (MQ=255)
                            atgaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATaa                                   <  1:676110/68‑1 (MQ=255)
                            atgaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAAt                                  >  1:902333/1‑69 (MQ=255)
                            atgaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATg                                 >  1:266262/1‑70 (MQ=255)
                            atgaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATg                                 <  1:1017611/70‑1 (MQ=255)
                             tgaACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGga                               <  1:1354425/71‑1 (MQ=255)
                                 ccAGATAGTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGAt                                                 >  1:943524/1‑49 (MQ=255)
                                   aGATAGTCAAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGa                          >  1:1527230/1‑70 (MQ=255)
                                        gTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCg                     <  1:1305142/70‑1 (MQ=255)
                                        gTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGa                    <  1:356065/71‑1 (MQ=255)
                                        gTCCAGACCGCCAGCAACCTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGa                    <  1:462697/71‑1 (MQ=255)
                                               ccGCCAGCAACCTGCATGGC‑‑ATAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGa              <  1:965463/70‑1 (MQ=255)
                                                         ccTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAAt                                  >  1:198348/1‑40 (MQ=37)
                                                         ccTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGAt                            <  1:784981/46‑1 (MQ=37)
                                                          cTGCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGtt  >  1:1314904/1‑71 (MQ=255)
                                                           tGCATGGCAG‑‑AAATAGCGGCGATACCCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGtt  >  1:724280/1‑70 (MQ=255)
                                                            gCATGGCAG‑‑AAATAGCGGCGATAACCGCCATGATaa                                   <  1:191309/36‑1 (MQ=25)
                                                                     ||                                                            
GGTTACGGCGCAGCAGCTTTTCTGTCTGATGAACCAGATAGTCCAGACCGCCAGCAACCTGCATGGCAGAAAAATAGCGGCGATAACCGCCATGATAATGGAGATGACATCGAACGGGATGTTACCGGGTT  >  W3110S.gb/4371204‑4371334

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑