Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 4,310,365 Δ1,199 bp 100% insH[alsK] insH, [alsK]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 4310365–4311562 4311563 2–1199 34 [0] [0] 34 insH–[alsK] insH,[alsK]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 43103640 (0.000)29 (1.690) 20/92 NT 100% intergenic (‑286/‑69) yjcS/insH predicted alkyl sulfatase/IS5 element protein
?W3110S.gb 4311564 = 0 (0.000)coding (916/1029 nt) alsK D‑allose kinase

ATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/4310291‑4310364
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttagCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAG  >  W3110S.gb/4311564‑4311632
                                                                                                                                               
ATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCAAGGGGCTT                                                                          >  1:157793/1‑71
      ATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCA                                                                     >  1:126345/1‑70
             CTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTG                                                             <  1:927606/71‑1
                TCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGG                                                          >  1:1360898/1‑71
                      GCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAA                                                     >  1:1748218/1‑70
                         CAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACG                                                 <  1:913114/71‑1
                          AGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGT                                                <  1:296559/71‑1
                           GCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTT                                               <  1:684255/71‑1
                           GCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTT                                               <  1:467141/71‑1
                           GCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTT                                               <  1:1549113/71‑1
                           GCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGT                                                <  1:905215/70‑1
                                agATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGC                                          >  1:1002822/3‑71
                                    TGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAA                                                     >  1:861501/1‑56
                                     GCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAA                                     >  1:676467/1‑71
                                     GCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAA                                     >  1:403361/1‑71
                                     GCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGA                                             >  1:292926/1‑63
                                      caCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAA                                     <  1:51608/68‑1
                                      CGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAA                                     <  1:556275/70‑1
                                       GCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGA                                             >  1:1345075/1‑61
                                       GCTACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGA                                             >  1:588559/1‑61
                                         TACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATT                                 >  1:1237159/1‑71
                                          ACTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTG                                >  1:1177953/1‑71
                                           CTGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTG                                <  1:431056/70‑1
                                            TGTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCC                                       <  1:46777/62‑1
                                             GTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGT                                                >  1:1237171/1‑52
                                             GTGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGT                                                >  1:378541/1‑52
                                              TGCCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCT                              >  1:13777/1‑69
                                                CCAACGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCAC                          >  1:418011/1‑71
                                                    CGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACC                         <  1:1097974/68‑1
                                                    CGTTTTACTCATGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAAT                                    <  1:1536528/57‑1
                                                               TGGGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTA            <  1:1635233/70‑1
                                                                 GGGCTTTAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAG         >  1:100158/1‑71
                                                                      TTAGCACAGAACTGTGGCAATAAACGTTGATGCGCCAATTTTGCTGCACCCTGAGCGCCATTAAAGTCAG     <  1:1096739/70‑1
                                                                       TAGCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAG     <  1:1026528/69‑1
                                                                         GCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAG  <  1:590150/70‑1
                                                                                                                                               
ATATATATTGTACCTGTCGTTGGCACAGCGGGACATTGCGCTACTGTGCCAACGTTTTACTCATGGGGCTTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/4310291‑4310364
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttagCACAGAACTGTGGCAAAAAACGTTGATGCGCCAATATTGCTGCACCCTGAGCGCCATTAAAGTCAGAAG  >  W3110S.gb/4311564‑4311632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.