| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 47,858 | G→A | 64.0% | S30S (TCG→TCA) | kefC → | potassium:proton antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 47,858 | 0 | G | A | 64.0% | 17.0 / 23.3 | 25 | S30S (TCG→TCA) | kefC | potassium:proton antiporter |
| Reads supporting (aligned to +/- strand): ref base G (3/6); new base A (6/10); total (9/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 | |||||||||||
ATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTGACCG > W3110S.gb/47799‑47919 | aTTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTa < 1:1662495/71‑1 (MQ=255) ggTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGCGATCAGTACTTGGCTACCTGATc < 1:446536/69‑1 (MQ=255) gTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGcc < 1:302987/71‑1 (MQ=255) gcTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCACCGGCTGCatta < 1:736355/71‑1 (MQ=255) tGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGCCGGCTGCattatt > 1:1545180/1‑71 (MQ=255) gATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTAc < 1:676235/41‑1 (MQ=255) aTTGTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGcc > 1:968820/1‑57 (MQ=255) aTTGTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGCCGGCTGCATTATTg < 1:1480010/70‑1 (MQ=255) tGTACCCATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGcc > 1:287570/1‑71 (MQ=255) tACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCg < 1:1251536/71‑1 (MQ=255) tACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCg < 1:490828/71‑1 (MQ=255) cccATTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGccc < 1:168341/68‑1 (MQ=255) ccATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTgg > 1:977467/1‑71 (MQ=255) aTTGCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGCCg < 1:694908/49‑1 (MQ=255) gCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGc < 1:552722/70‑1 (MQ=255) gCGGTACGTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCt > 1:1400790/1‑71 (MQ=255) gTCTTGGTCTGGGATCAGTACTTGGCTACCTGATCGCCGGCTGCat < 1:839134/46‑1 (MQ=255) tCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGa > 1:626610/1‑67 (MQ=255) ggTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTg < 1:15632/69‑1 (MQ=255) ggTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTGAc > 1:1633907/1‑71 (MQ=255) ggTCTGGGATCAGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTGa > 1:192992/1‑70 (MQ=255) gTCTGGGATCAGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGAc > 1:274201/1‑63 (MQ=255) tCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTGACCg < 1:1121459/71‑1 (MQ=255) tCTGGGATCAGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCt < 1:660856/57‑1 (MQ=255) tCTGGGATCAGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTGACCg < 1:1617151/71‑1 (MQ=255) | ATTTATCTCGGTTCGGCAGCGCTGATTGTACCCATTGCGGTACGTCTTGGTCTGGGATCGGTACTTGGCTACCTGATCGCCGGCTGCATTATTGGCCCGTGGGGGCTGCGACTGGTGACCG > W3110S.gb/47799‑47919 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |