| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,639,730 | T→C | 41.7% | K222R (AAA→AGA) | xerC ← | site‑specific tyrosine recombinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,639,730 | 0 | T | C | 41.7% | 7.3 / 25.9 | 24 | K222R (AAA→AGA) | xerC | site‑specific tyrosine recombinase |
| Reads supporting (aligned to +/- strand): ref base T (9/5); new base C (2/8); total (11/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.72e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 | |||||||||||
ATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCTTCGC > W3110S.gb/3639671‑3639793 | aTTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTa > 1:1071168/1‑71 (MQ=255) tATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTAcg > 1:1624103/1‑70 (MQ=255) aTGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACAt < 1:834414/59‑1 (MQ=255) gCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTc < 1:474966/71‑1 (MQ=255) ccTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGa < 1:1078034/71‑1 (MQ=255) cTTGTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGa > 1:674155/1‑70 (MQ=255) ttGTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACATTACGCGCGga < 1:1364490/48‑1 (MQ=255) tGTTTTATGCCTCATTCGGCAAAGCGTTTCTGcaca > 1:1548061/1‑36 (MQ=255) tGTTTTATGCCCCATTCGGCAAAGCGTTTCTGcaca > 1:1586761/1‑36 (MQ=255) tGTTTTATGCCCCATTCGGCAAAGCGTTTCTGcaca > 1:246481/1‑36 (MQ=255) gTTTTATGCCCCATTCGGCAAAGCGTCTCTGCACAt > 1:780555/1‑36 (MQ=255) ttttATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGa < 1:1497732/70‑1 (MQ=255) ttttATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGa < 1:1112088/70‑1 (MQ=255) ttATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGaaa > 1:1006995/1‑70 (MQ=255) tATGCCCCATTCGGCAAAGCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGaaaa < 1:692224/70‑1 (MQ=255) aTGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGa > 1:357326/1‑62 (MQ=255) cccATTCGGCAAAGCGTCTCTGCACATTACGCGCGGAGATTCGCTTGCCCAGTTTCGACAGAAATAGcgcg < 1:954908/71‑1 (MQ=255) cccATTCGGCAAAGCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGcgcg < 1:156572/71‑1 (MQ=255) cATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGcgtc > 1:534266/1‑71 (MQ=255) ggCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCtt < 1:8705/71‑1 (MQ=255) ggCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCtt < 1:1017439/71‑1 (MQ=255) ggCAAAGCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCtt < 1:313740/71‑1 (MQ=255) aaaGCGTCTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCTTCGc < 1:635468/71‑1 (MQ=255) aGCGTTTCTGCACATTACGCGCGGAGAAACGCTTGCCCAGTTTCGACAGAAAAAGCGcgt > 1:1215344/1‑60 (MQ=255) | ATTTATGCGGATGAACGTGATTATTCAGCCCTTGTTTTATGCCCCATTCGGCAAAGCGTTTCTGCACATTACGCGCGGAGATACGCTTGCCCAGTTTCGACAGAAAAAGCGCGTCGTCTTCGC > W3110S.gb/3639671‑3639793 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |