| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,704,876 | C→T | 57.1% | M164I (ATG→ATA) | trkD ← | potassium transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,704,876 | 0 | C | T | 57.1% | 3.1 / 24.0 | 21 | M164I (ATG→ATA) | trkD | potassium transporter |
| Reads supporting (aligned to +/- strand): ref base C (5/4); new base T (7/5); total (12/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGTAACCCCAGTCCTGCCAGAATCAAAAACCAGGTCAGCATGATCGGCGCAAACAGCTTACCGACCATAGCCGTGCCATGTTTTTGAATCATAAATAATAAAGTGAGAACGATAATTGAGAGG > W3110S.gb/3704811‑3704933 | cGTAACCCCAGTCCTGCCAGAATCAAAAACCAGGTCAGCATGATCGGCGCAAACAGCTTACCGACtata < 1:996380/69‑1 (MQ=255)cGTAACCCCAGTCCTGCCAGAATCAAAAACCAGGTCAGCATGATCGGCGCAAACAGCTTACCGACtata < 1:1538098/69‑1 (MQ=255) gTAACCCCAGTCCTGCCAGAATCAAAAACCAGGTCAGCATGATCGGCGCAAACAGCTTACCGACTATAGcc < 1:1724470/71‑1 (MQ=255) gTCCTGCCAGAATCAAAAACCAGGTCAGCATGATCGGCGCAAACAGCTTACCGACTATAGCCGTGCCATGt < 1:1264188/71‑1 (MQ=255) tCCTGCCAGAATCAAAAACCAGGTCAGCATGATCGGCGCAAACAGCTTACCGACTATAGCCGTg < 1:1132189/64‑1 (MQ=255) ccAGAATCAAAAACCAGGTCAGCATGATCGGCGCAAACAGCTTACCGACTATAGCCGTGCCATGTTTTTGa < 1:953260/71‑1 (MQ=255) aaTCAAAAACCAGGTCAGCATGATCGGCGCAAACAGCTTACCGACCATAGCCGTGCCATGTTTTTGAATc < 1:315359/70‑1 (MQ=255) tCAAAAACCAGGTCAGCATGATCGGCGCAAACAGCTTACCGACCATAGCCGTGCCATGTTTTTGAATCat > 1:862085/1‑70 (MQ=255) tCAAAAACCAGGTCAGCATGATCGGCGCAAACAGCTTACCGACCATAGCCGTGCCATGTTTTTGAATCat > 1:511747/1‑70 (MQ=255) aaaCCAGGTCAGCATGATCGGCGCAAACAGCTTACCGACTATAGCCGTGCCATGTTTTTGAATCataaa > 1:405230/1‑69 (MQ=255) aaaCCAGGTCAGCATGATCGGCGCAAACAGCTTACCGACCATAGCCGTGCCATGTTTTTGAATCataaata < 1:1298394/71‑1 (MQ=255) aGCATGATCGGCGCAAACAGCTTACCGACCATAGCCGTGCCATGTTTTTGAATCATAAATAATAAAGt > 1:952619/1‑68 (MQ=255) aTGATCGGCGCAAACAGCTTACCGACTATAGCCGTGCCATGTTTTTGAATCa < 1:941009/52‑1 (MQ=255) ggCGCAAACAGCTTACCGACCATAGCCGTGCCATGTTTTTGAATCataa > 1:1336017/1‑49 (MQ=255) ggCGCAAACAGCTTACCGACCATAGCCGTGCCATGTTTTTGAATCATAAATAATAAAGTGAGAACGATAAt > 1:1675299/1‑71 (MQ=255) aaCAGCTTACCGACTATAGCCGTGCCATGTTTTTGAATCATAAATAATAAAGTGAGAACGATAATTga > 1:1635169/1‑68 (MQ=255) aaCAGCTTACCGACTATAGCCGTGCCATGTTTTTGAATCATAAATAATAAAGTGAGAACGATAATTga > 1:1054571/1‑68 (MQ=255) aaCAGCTTACCGACCATAGCCGTGCCATGTTTTTGAATCATAAATAATAAAGTGAGAACGATAAt < 1:1198336/65‑1 (MQ=255) aCAGCTTACCGACTATAGCCGTGCCATGTTTTTGAATCATAAATAATaaa > 1:988874/1‑50 (MQ=255) aCAGCTTACCGACTATAGCCGTGCCATGTTTTTGAATCATAAATAATAAAGTGAGAACGATAATTGAGAgg > 1:63037/1‑71 (MQ=255) aCAGCTTACCGACTATAGCCGTGCCATGTTTTTGAATCATAAATAATAAAGTGAGAACGATAATTGAGAgg > 1:639246/1‑71 (MQ=255) cAGCTTACCGACTATAGCCGTGCCATGTTTTTGaa > 1:417825/1‑35 (MQ=255) cTTACCGACCATAGCCGTGCCATGTTTTTGAATCataaat < 1:197464/40‑1 (MQ=255) | CGTAACCCCAGTCCTGCCAGAATCAAAAACCAGGTCAGCATGATCGGCGCAAACAGCTTACCGACCATAGCCGTGCCATGTTTTTGAATCATAAATAATAAAGTGAGAACGATAATTGAGAGG > W3110S.gb/3704811‑3704933 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |