| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,707,036 | G→A | 44.4% | A483T (GCC→ACC) | yieN → | fused predicted transcriptional regulators |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,707,036 | 0 | G | A | 44.4% | 9.4 / 29.8 | 27 | A483T (GCC→ACC) | yieN | fused predicted transcriptional regulators |
| Reads supporting (aligned to +/- strand): ref base G (8/7); new base A (6/6); total (14/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACGCTTTATTCAGCGAACAGCAAAAATGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAAGCCAGCCTACAGGATGTCGGTGCACAGATTCGCCAGGCACAACAATGCTAACGCTGGATACGCTT > W3110S.gb/3706970‑3707100 | cacGCTTTATTCAGCGAACAGCAAAAATGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAagccag > 1:101334/1‑71 (MQ=255)cacGCTTTATTCAGCGAACAGCAAAAATGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAagccag > 1:647271/1‑71 (MQ=255) cTTTATTCAGCGAACAGCAAAAATGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAAGCCAGCCTa > 1:1468214/1‑71 (MQ=255) aTTCAGCGAACAGCAAAAATGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAAACCAGCCTACAg > 1:1588646/1‑70 (MQ=255) cAGCGAACAGCAAAAATGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAAACCAGCCTACAGGATg > 1:1565413/1‑71 (MQ=255) gCGAACAGCAAAAATGTCTGTTTATCCCTGGCGACGGGTTAGGTCGCATTGAAGCCAGCCTACAGGATGTc < 1:1315412/71‑1 (MQ=255) cGAACAGCAAAAATGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAAACCAGCCTACAGGATGTCg < 1:305840/71‑1 (MQ=255) gAACAGCAAAAATGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAAGCCAGCCTACAGGATGt < 1:1745909/68‑1 (MQ=255) aCATCAAAAATGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAAACCAGCCTACAGGATGTCGGTg < 1:1289268/71‑1 (MQ=255) cAGCAAAAATGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAAACCAGCCTACAGGATGTCGGTGc < 1:748327/71‑1 (MQ=255) cAAAAATGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAAGCCAGCCTACAGGATGTCGGTGca > 1:1017069/1‑69 (MQ=255) cAAAAATGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAAGCCAGCCTACAGGATGTCGGTGca > 1:128778/1‑69 (MQ=255) cAAAAATGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAAACCAGCCTACAGGATGTCGGTGca > 1:1762095/1‑69 (MQ=255) aaaaTGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAAGCCAGCCTACAGGATGTCGGt < 1:760785/64‑1 (MQ=255) aaaaTGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAAGCCAGCCTACAGGATGTCGGTGca > 1:1690338/1‑67 (MQ=255) tGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAAGCCAGCCTACAGGATGTCGGTGCACAGATTCg > 1:1269816/1‑71 (MQ=255) tGTCTGTTTATCCCTGGCGACTGGTTAGGACGCATTGAAACCAGCCTACAGGATGTCGGTGCACAGATTCg > 1:584072/1‑71 (MQ=255) cTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAAGCCAGCCTAc < 1:336465/46‑1 (MQ=255) tGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAAACCAGCCTACAGGATGTCGGTGCACAGa < 1:1617606/63‑1 (MQ=255) tttATCCCTGGCGACTGGTTAGGTCGCATTGAAACCAGCCTACAGGATGTCGGTGCACAGAtt > 1:819091/1‑63 (MQ=255) cTGGCGACTGGTTAGGTCGCATTGAAGCCAGCCTACAg < 1:1329379/38‑1 (MQ=255) ggCGACTGGTTAGGTCGCATTGAAGCCAGCCTACAGGATGTCGGTGCACAGATTCGCCAGGCACAACAAt > 1:1226871/1‑70 (MQ=255) cTGGTTAGGTCGCATTGAAGCCAGCCTACAGGATGTCGGTGCACAGATTCGCCAGGCACAACAATGCTAAc > 1:1229372/1‑71 (MQ=255) cTGGTTAGGTCGCATTGAAACCAGCCTACAGGATGTCGGTGCACAGATTCGCCAGGCACAACAATGCTAAc < 1:1132511/71‑1 (MQ=255) ggTCGCATTGAAACCAGCCTACAGGATGTCGGTGCACAGATTCGCCAGGCACAACAATGCTAACGCTGGa < 1:557638/70‑1 (MQ=255) gCATTGAAGCCAGCCTACAGGATGTCGGTGCACAGATTCGCCAGGCACAACAATGCTAACGCTGGATACGc > 1:112826/1‑71 (MQ=255) ttGAAGCCAGCCTACAGGATGTCGGTGCACAGATTCGc < 1:1357504/38‑1 (MQ=255) ttGAAGCCAGCCTACAGGATGTCGGTGCACAGATTCGCCAGGCACAACAATGCTAACGCTGGATACGCtt < 1:450722/70‑1 (MQ=255) gAAACCAGCCTACAGGATGTCGGTGCACAGATTCGc > 1:617951/1‑36 (MQ=255) | CACGCTTTATTCAGCGAACAGCAAAAATGTCTGTTTATCCCTGGCGACTGGTTAGGTCGCATTGAAGCCAGCCTACAGGATGTCGGTGCACAGATTCGCCAGGCACAACAATGCTAACGCTGGATACGCTT > W3110S.gb/3706970‑3707100 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |