| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,727,747 | T→C | 38.9% | V168V (GTT→GTC) | pstA → | phosphate transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,727,747 | 0 | T | C | 38.9% | 7.2 / 17.1 | 18 | V168V (GTT→GTC) | pstA | phosphate transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (7/4); new base C (4/3); total (11/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCACTTCTCCGGCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGAAGCGGCTTA > W3110S.gb/3727690‑3727806 | gCACTTCTCCGGCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTTATCCGCACCACCg < 1:1397400/71‑1 (MQ=255) ttCTCCGGCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTCATCCGCACCACCGAGaa > 1:1608783/1‑71 (MQ=255) tctcCGGCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTCATCCGcac > 1:238474/1‑61 (MQ=255) ctcCGGCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTTATCCGCACCACCGAGAACa > 1:1279897/1‑71 (MQ=255) ctcCGGCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTTATCCGCACCACCGAGAACa > 1:453713/1‑71 (MQ=255) ctcCGGCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTTATACGCACCACCGAGAACa > 1:911507/1‑71 (MQ=255) ctcCGGCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTCATCCGCACCACCGAGAAc < 1:11513/70‑1 (MQ=255) tcCGGCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTTATCCGCACCACCGAGAACAt > 1:1386312/1‑71 (MQ=255) ggcgggcgTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAAc < 1:1487710/71‑1 (MQ=255) aTTGCCCTGGCGTTGTTGCAGGTGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCTTa > 1:15943/1‑71 (MQ=255) gCGTTGTTGCAGGTGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTg < 1:787721/69‑1 (MQ=255) ttgttgCAGGTGCCGATTGTCATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGa < 1:1410925/71‑1 (MQ=255) ttgttgCAGGTGCCGATTGTCATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGa < 1:891946/71‑1 (MQ=255) gCAGGTGCCGATTGTCATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGAAGCg > 1:1238368/1‑70 (MQ=255) cAGGTGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGACGTACAGCCTGCGTGAAGCgg < 1:1152172/70‑1 (MQ=255) aGGTGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGAAGCGGCt > 1:704794/1‑71 (MQ=255) aGGTGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGAAGCGGCt > 1:987821/1‑71 (MQ=255) gCCGATTGTCATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGAAGCGGCTTa > 1:1308619/1‑69 (MQ=255) | GCACTTCTCCGGCTGGGCGGGCGTGATTGCCCTGGCGTTGTTGCAGGTGCCGATTGTTATCCGCACCACCGAGAACATGCTGAAACTGGTGCCGTACAGCCTGCGTGAAGCGGCTTA > W3110S.gb/3727690‑3727806 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |