Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	3,739,327	C→T	51.4%	V195I (GTT→ATT)	yieH ←	predicted hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,739,327	0	C	T	51.4%	‑4.6 / 53.4	37	V195I (GTT→ATT)	yieH	predicted hydrolase
Reads supporting (aligned to +/- strand):  ref base C (8/10);  new base T (11/8);  total (19/18)
Fisher's exact test for biased strand distribution p-value = 5.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACCACGCGCTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCGATAC  >  W3110S.gb/3739262‑3739386                                                                  |                                                            aCCACGCGCTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCg                                                        >  1:868775/1‑71 (MQ=255)       cgcTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGAt                                                          >  1:663823/1‑63 (MQ=255)       cgcTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGAt                                                          >  1:1432473/1‑63 (MQ=255)       cgcATTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGAt                                                          >  1:666042/1‑63 (MQ=255)            tCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGc                                             >  1:209617/1‑71 (MQ=255)              cacaGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCgg                                           >  1:459955/1‑71 (MQ=255)               acaGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCggg                                          >  1:453737/1‑71 (MQ=255)                caGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGt                                         >  1:1728183/1‑71 (MQ=255)                 aGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTc                                        >  1:548040/1‑71 (MQ=255)                 aGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTc                                        >  1:525293/1‑71 (MQ=255)                  gTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTc                                        <  1:1402395/70‑1 (MQ=255)                        gTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAg                                 <  1:1225033/71‑1 (MQ=255)                         tAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAg                                 >  1:21731/1‑70 (MQ=255)                          aaCTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCa                                  >  1:320785/1‑68 (MQ=255)                             tGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAAc                                                             <  1:859609/38‑1 (MQ=255)                             tGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAAc                                                             <  1:1722871/38‑1 (MQ=255)                               cGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTa                            <  1:1349513/69‑1 (MQ=255)                                gAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAg                              <  1:338173/66‑1 (MQ=255)                                     aTGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACtt                    <  1:1010321/71‑1 (MQ=255)                                      tGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGt                                         >  1:376739/1‑49 (MQ=255)                                       gggTAAAGGTGGTGACTTTCGGGTGAACGATCGGCt                                                     <  1:1148260/36‑1 (MQ=255)                                       gggTAAAGGTGGTGACTTTCGGGTGAACGATCGGCt                                                     <  1:635043/36‑1 (MQ=255)                                       gggTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAaca                       >  1:1511984/1‑66 (MQ=255)                                        ggTAAAGGTGGTGACTTTCGGGTGAACGATCGGCt                                                     <  1:1146101/35‑1 (MQ=255)                                         gTAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAg                           <  1:57999/60‑1 (MQ=255)                                          tAAAGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTc                   <  1:1414235/67‑1 (MQ=255)                                            aaGGTGGTGACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATAc              <  1:1599631/70‑1 (MQ=255)                                               gtggtgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGaa                         >  1:290849/1‑56 (MQ=255)                                                tggtgACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCTCAGAAGTAGAac                        <  1:598375/56‑1 (MQ=255)                                                tggtgACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAac                        <  1:751928/56‑1 (MQ=255)                                                  gtgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGaa                         <  1:1655500/53‑1 (MQ=255)                                                   tgACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTc       <  1:1647294/70‑1 (MQ=255)                                                    gACTTTCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTc       >  1:1279538/1‑69 (MQ=255)                                                     aCTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCGa     <  1:561704/70‑1 (MQ=255)                                                     aCTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCGa     <  1:1006195/70‑1 (MQ=255)                                                       tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCa                 >  1:447232/1‑56 (MQ=255)                                                       tttCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTc       <  1:1639455/66‑1 (MQ=255)                                                         tCGGGTGAATGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCGATAc  >  1:167769/1‑69 (MQ=255)                                                         tCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGaa                         >  1:465145/1‑46 (MQ=255)                                                                  |                                                            ACCACGCGCTTTCCACAGTTCAGGTAACTGCGAAAGATGGGTAAAGGTGGTGACTTTCGGGTGAACGATCGGCTTATTGTGCGGGTCGGCGCAGAAGTAGAACACTTCCATACCTGCGTCGATAC  >  W3110S.gb/3739262‑3739386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑