| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 113,035 | T→C | 17.4% | D62G (GAT→GGT) | coaE ← | dephospho‑CoA kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 113,035 | 0 | T | C | 17.4% | 49.9 / 5.7 | 23 | D62G (GAT→GGT) | coaE | dephospho‑CoA kinase |
| Reads supporting (aligned to +/- strand): ref base T (14/5); new base C (2/2); total (16/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.48e-01 | |||||||||||
GTTAAGCCAGTTTTTCTCTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAAC > W3110S.gb/112965‑113102 | gTTAAGCCAGTTTTTCTCTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCAt > 1:1024525/1‑71 (MQ=255) aaGCCAGTTTTTCTCTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACcag > 1:1319933/1‑71 (MQ=255) aaGCCAGTTTTTCTCTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACcag > 1:213250/1‑71 (MQ=255) gCCAGTTTTTCTCTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCATcag > 1:1541587/1‑69 (MQ=255) aGTTTTTCTCTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCATCagcagc > 1:807076/1‑70 (MQ=255) tttttCTCTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt < 1:670536/71‑1 (MQ=255) tttttCTCTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCACCAGCAGCAAt < 1:1736118/71‑1 (MQ=255) ccGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAgcg > 1:588241/1‑71 (MQ=255) ggCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGt > 1:7539/1‑71 (MQ=255) gCTCGCGCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAAt > 1:15329/1‑70 (MQ=255) cTCGCGCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAg < 1:394216/58‑1 (MQ=255) cTCGCGCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATgg < 1:1276050/71‑1 (MQ=255) tCGCGCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCAAAAGTGAt > 1:1744413/1‑61 (MQ=255) cAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCAt > 1:908183/1‑68 (MQ=255) ggCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAgcg > 1:1376974/1‑71 (MQ=255) gCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAgcgc < 1:700912/71‑1 (MQ=255) cccGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAgcg > 1:1412521/1‑69 (MQ=255) cccGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAgcg > 1:1212348/1‑69 (MQ=255) tGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGAGCAGGTGCACCTGGtt < 1:1051743/71‑1 (MQ=255) gTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAg > 1:116719/1‑40 (MQ=255) ttCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTc > 1:1580062/1‑70 (MQ=255) ccATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTACAac > 1:743591/1‑71 (MQ=255) aTCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATg < 1:1306197/45‑1 (MQ=255) aTCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAac > 1:23876/1‑69 (MQ=255) | GTTAAGCCAGTTTTTCTCTTCCGGGTTGGCGAAGATCCGCTCGCGCAAGGCCCGGCGCTGCAATGTTCCATCAGCAGCAATCATGTTAGCGCCAAAGTGATCAGCAATGGCATGTAGCGCAGGTGCACCTGGTTCAAC > W3110S.gb/112965‑113102 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |