| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,783,789 | C→A | 28.5% | D72Y (GAC→TAC) | yidI ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,783,789 | 0 | C | A | 28.5% | 44.7 / 8.1 | 21 | D72Y (GAC→TAC) | yidI | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (5/10); new base A (3/3); total (8/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAAGTAGCGCAGCAATGGCCAGTGCGATACCGCTTTGATTGAGGATTTTATCGACATCTACGTCCTCTTTTTTCTCCACGGTGGTAATTTTTTCGCCTTTTATTCCGGCGAGAAGACCTCTTTTAATT > W3110S.gb/3783726‑3783853 | aaaGTAGCGCAGCAATGGCCAGTGCGATACCGCTTTGATTGAGGATTTTATCGACATCTACGTACTCtttt > 1:1150126/1‑71 (MQ=255) aaGTAGCGCAGCAATGGCCAGTGCGATACCGCTTTGATTGAGGATTTTATCGACATCTACGTCCTCtttt < 1:1045318/70‑1 (MQ=255) gcagcaATGGCCAGTGCGATACCGCTTTGATTGAGGATTTTATCGACATCTACGTCCTCTTTTTTCTCCAc < 1:837753/71‑1 (MQ=255) agcaATGGCCAGTGCGATACCGCTTTGATTGAGGATTTTATCGACATCTACGTCCTCTTTTTTCTCCACgg < 1:815320/71‑1 (MQ=255) aTGGCCAGTGCGATACCGCTTTGATTGAGGATTTTATCGACATCTACGTCCTCtttttt > 1:1325287/1‑59 (MQ=255) aTGGCCAGTGCGATACCGCTTTGATTGAGGATTTTATCGACATCTACGTCCTCtttttt > 1:177343/1‑59 (MQ=255) tGGCCAGTGCGATACCGCTTTGATTGAGGATTTTATCGACATCTACGTCCTCtttttt < 1:1256160/58‑1 (MQ=255) gTGCGATACCGCTTTGATTGAGGATTTTATCGACATCTACGTCCTCTTTTTTCTCCACGGTGGTaa < 1:1643021/66‑1 (MQ=255) gTGCGATACCGCTTTGATTGAGGATTTTATCGACATCTACGTCCTCTTTTTTCTCCACGGTGGTaa < 1:521913/66‑1 (MQ=255) gTGCGATACCGCTTTGATTGAGGATTTTATCGACATCTACGTACTCTTTTTTCTCCACGGTGGTAAtttt < 1:1089239/70‑1 (MQ=255) gTGCGATACCGCTTTGATTGAGGATTTTATCGACATCTACGTACTCTTTTTTCTCCACGGTGGTAAtttt < 1:379345/70‑1 (MQ=255) aCCGCTTTGATTGAGGATTTTATCGACATCTACGTCCTCttt < 1:1004118/42‑1 (MQ=255) gCTTTGATTGAGGATTTTATCGACATCTACGTCCTCTTTTTTCTCCACGGTGGTAATTTTTTCGCCTTTTa < 1:268374/71‑1 (MQ=255) tgattgaGGATTTTATCGACATCTACGTACTCtttt < 1:13997/36‑1 (MQ=255) gattgaGGATTTTATCGACATCTACGTACTCTTTTTTCTCCACgg > 1:294131/1‑45 (MQ=255) aTTTTATCGACAGCTACGTCCTCTTTTTTCTCCACGGTGGTAATTTTTTCGCCTTTTATTCCGGCGagaag < 1:1765539/71‑1 (MQ=255) tttATCGACATCTACGTCCTCTTTTTTCTCCACGGTGGTAATTTTTTCGCCtt < 1:811770/53‑1 (MQ=255) tATCGACATCTACGTACTCTTTTTTCTCCACGGTGGTAATTTTTTCGCCTTTTATTCCGGCGAGAAGACct > 1:55516/1‑71 (MQ=255) cTACGTCCTCTTTTTTCTCCACGGTGGTAATTTTTTCGCCTTTTa > 1:649601/1‑45 (MQ=255) tACGTCCTCTTTTTTCTCCACGGTGGTAATTTTTTCGCCTTTTATTcc > 1:769530/1‑48 (MQ=255) tACGTCCTCTTTTTTCTCCACGGTGGTAATTTTTTCGCCTTTTATTCCGGCGAGAAGACCTCTTTTAAtt > 1:1194531/1‑70 (MQ=255) | AAAGTAGCGCAGCAATGGCCAGTGCGATACCGCTTTGATTGAGGATTTTATCGACATCTACGTCCTCTTTTTTCTCCACGGTGGTAATTTTTTCGCCTTTTATTCCGGCGAGAAGACCTCTTTTAATT > W3110S.gb/3783726‑3783853 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |