Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,824,514 T→C 100% pseudogene (647/687 nt) rph → ECK3633:JW3618:b3643; ribonuclease PH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,824,5140TC100.0% 50.0 / NA 16pseudogene (647/687 nt)rphECK3633:JW3618:b3643; ribonuclease PH
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/10);  total (6/10)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGGCGCATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCATCTTGTTGGCTCTGGCCCGA‑GGGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGC  >  W3110S.gb/3824450‑3824572
                                                                |                                                           
gggCGCATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCttgtt                                                       >  1:591710/1‑71 (MQ=255)
        catTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTgg                                               >  1:925300/1‑71 (MQ=255)
            gAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc                                            <  1:1466905/70‑1 (MQ=255)
            gAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc                                            <  1:896245/70‑1 (MQ=255)
             aaGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCg                                           <  1:1592851/70‑1 (MQ=255)
                       gACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcg                                <  1:897506/71‑1 (MQ=255)
                         cGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcgaa                              >  1:1556389/1‑71 (MQ=255)
                             aGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCa                          <  1:704075/71‑1 (MQ=255)
                                            cACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g                                        >  1:1061868/1‑41 (MQ=38)
                                              cccATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcg                                <  1:103367/48‑1 (MQ=255)
                                              cccATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAGAAg         >  1:1211786/1‑71 (MQ=255)
                                               ccATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAGAAgg        <  1:1530249/71‑1 (MQ=255)
                                                  tGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcg                                <  1:1425942/44‑1 (MQ=25)
                                                  tGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGgaatcg                                <  1:908625/44‑1 (MQ=25)
                                                     agagCTACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCATTGTAGCGACGCAGAAGGCGgcgc  >  1:1337977/1‑71 (MQ=255)
                                                          tACTCACCTTGTTGGCGCTGGCCCGAGGGGGAATCGAATCCAt                         <  1:1414918/43‑1 (MQ=25)
                                                                |                                                           
GGGCGCATCATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCATCTTGTTGGCTCTGGCCCGA‑GGGGAATCGAATCCATTGTAGCGACGCAGAAGGCGGCGC  >  W3110S.gb/3824450‑3824572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑