| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,824,524 | T→G | 100% | pseudogene (657/687 nt) | rph → | ECK3633:JW3618:b3643; ribonuclease PH |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,824,524 | 0 | T | G | 100.0% | 50.7 / NA | 16 | pseudogene (657/687 nt) | rph | ECK3633:JW3618:b3643; ribonuclease PH |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (6/10); total (6/10) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCATCTTGTTGGCTCTGGCCCGA‑GGGG‑AATCGAATCCATTGTAGCGACGCAGAAGGCGGCGC > W3110S.gb/3824458‑3824572 | catTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTgg > 1:925300/1‑71 (MQ=255) gAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc < 1:1466905/70‑1 (MQ=255) gAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGccc < 1:896245/70‑1 (MQ=255) aaGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCg < 1:1592851/70‑1 (MQ=255) gACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGg‑aatcg < 1:897506/71‑1 (MQ=255) cGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGg‑aatcgaa > 1:1556389/1‑71 (MQ=255) aGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCa < 1:704075/71‑1 (MQ=255) cACCCATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGA‑g > 1:1061868/1‑41 (MQ=38) cccATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGg‑aatcg < 1:103367/48‑1 (MQ=255) cccATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCAGAAg > 1:1211786/1‑71 (MQ=255) ccATGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCAGAAgg < 1:1530249/71‑1 (MQ=255) tGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGg‑aatcg < 1:1425942/44‑1 (MQ=25) tGAAGAGCTACTCACCTTGTTGGCGCTGGCCCGAGGGGg‑aatcg < 1:908625/44‑1 (MQ=25) agagCTACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCATTGTAGCGACGCAGAAGGCGgcgc > 1:1337977/1‑71 (MQ=255) tACTCACCTTGTTGGCGCTGGCCCGAGGGGG‑AATCGAATCCAt < 1:1414918/43‑1 (MQ=25) tgttgGCGCTGGCCCGA‑GGGGAAATCGAATCCATTGTa > 1:19124/1‑38 (MQ=37) | CATTGAAGTGCAGGGGACGGCAGAAGGCGAGCCGTTCACCCATGAAGAGCTACTCATCTTGTTGGCTCTGGCCCGA‑GGGG‑AATCGAATCCATTGTAGCGACGCAGAAGGCGGCGC > W3110S.gb/3824458‑3824572 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |