| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,915,241 | A→G | 57.9% | E48G (GAG→GGG) | glyQ → | glycine tRNA synthetase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,915,241 | 0 | A | G | 57.9% | 6.3 / 19.3 | 19 | E48G (GAG→GGG) | glyQ | glycine tRNA synthetase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (3/5); new base G (8/3); total (11/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01 | |||||||||||
TTGTTCAACCATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCCCGACCGATGGTCGCTACG > W3110S.gb/3915178‑3915309 | ttGTTCAACCATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTgggg > 1:1629382/1‑71 (MQ=255)ttGTTCAACCATAGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTg < 1:1566620/68‑1 (MQ=255) cAACCATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGCCAg > 1:540437/1‑70 (MQ=255) cAACCATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTg < 1:599050/63‑1 (MQ=255) cATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATggcg > 1:1475591/1‑70 (MQ=255) ggCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATGGCGGCTGCTTa > 1:462976/1‑68 (MQ=255) gcgGGAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCa < 1:723107/71‑1 (MQ=255) ggAACCTCTCACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGcc < 1:323246/71‑1 (MQ=255) ctctCACCCAATGACCTGTCTGCGCGGGCTGGGGCCAGAAcc < 1:1134483/42‑1 (MQ=255) tctcACCCAATGACCTGTCTGCGCGAGCTGGGGCCAg > 1:1166689/1‑37 (MQ=255) tctcACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCt < 1:692478/66‑1 (MQ=255) tcACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCtcg < 1:1385039/69‑1 (MQ=255) cccAAAGACATGTCTGCGCGGGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGcc > 1:1387539/1‑71 (MQ=255) ccAATGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGccc > 1:428524/1‑71 (MQ=255) aTGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATgg > 1:1371584/1‑37 (MQ=255) aTGACCTGTCTGCGCGGGCTGGGGCCAGAACCGATgg > 1:994972/1‑37 (MQ=255) tCTGCGCGATCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCCCGACCGATgg < 1:907659/69‑1 (MQ=255) cgAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCCCGACCGATGGTCGCTACg > 1:143952/1‑71 (MQ=255) cgAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCCAGACCGATGGTCGCTa > 1:1196313/1‑69 (MQ=255) | TTGTTCAACCATTGGACATGGAAGTCGGCGCGGGAACCTCTCACCCAATGACCTGTCTGCGCGAGCTGGGGCCAGAACCGATGGCGGCTGCTTATGTTCAGCCTTCTCGTCGCCCGACCGATGGTCGCTACG > W3110S.gb/3915178‑3915309 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |