| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,986,216 | A→G | 13.3% | Y81H (TAT→CAT) | slp ← | outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,986,216 | 0 | A | G | 13.3% | 73.3 / 5.2 | 30 | Y81H (TAT→CAT) | slp | outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base A (14/12); new base G (2/2); total (16/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01 | |||||||||||
CAAGGAAGCCGCTTTGTCTGGCGAGCAGTCGGCCCTGATAGTTGGCTTCAATATCAGGCTTCGCATAGCTATCCAACGGTAATACAGAGATTTCTAACAACGTATCCGTTTTGCCATTGATAACGTTGATAACCTTC > W3110S.gb/3986150‑3986286 | cAAGGAAGCCGCTTTGTCTGGCGAGCAGTCGGCCCTGATAGTTGGCTTCAATATCAGGCTTCGCATGGCTa > 1:706317/1‑71 (MQ=255)cAAGGAAGCCGCTTTGTCTGGCGAGCAGTCGGCCCTGATAGTTGGCTTCAATATCAGGCTTCGCATAGCTa > 1:662216/1‑71 (MQ=255) gAAGCCGCTTTGTCTGGCGAGCAGTCGGCCCTGATAGTTGGCTTCAATATCAGGCTTCGCATGGCt < 1:1597512/66‑1 (MQ=255) aGCCGCTTTGTCTGGCGAGCAGTCGGCCCTGATAGTTGGCTTCAATATCAGGCTTCGCATAGCTATCCAAc > 1:581666/1‑71 (MQ=255) aGCCGCTTTGTCTGGCGAGCAGTCGGCCCTGATAGTTGGCTTCAATATCAGGCTTCGCATAGCTATCCAAc > 1:259561/1‑71 (MQ=255) gCTTTGTCTGGCGAGCAGTCGGCCCTGATAGTTGGCTTCAATATCAGGCTTCGCATAGCTATCCAACGGTa < 1:721170/71‑1 (MQ=255) cTTTGTCTGGCGAGCAGTCGGCCCTGATAGTTGGCTTCAATATCAGGCTTCGCATAGCTATCCAACGGTaa < 1:136715/71‑1 (MQ=255) tGTCTGGCGAGCAGTCGGCCCTGATAGTTGGCTTCAATATCAGGCTTCGCATAGCTATCCAACGGTAATa > 1:1218917/1‑70 (MQ=255) tGTCTGGCGAGCAGTCGGCCCTGATAGTTGGCTTCAATATCAGGCTTCGCATAGCTATCCAACGGTAATa < 1:467909/70‑1 (MQ=255) gCGAGCAGTCGGCCCTGATAGTTGGCTTCAATATCAGGCTTCGCATAGCTATCCAACGGTAATACAGAGAt > 1:218175/1‑71 (MQ=255) gCAGTCGGCCCTGATAGTTGGCTTCAATATCAGGCTTCGCATAGCTATCCAACGGTAATACAGAGATTTCt < 1:277543/71‑1 (MQ=255) gCAGTCGGCCCTGATAGTTGGCTTCAATATCAGGCTTCGCATAGCTATCCAACGGTAATACAGAGATTTCt < 1:882709/71‑1 (MQ=255) ctgCCCTGATAGTTGGCTTCAATATCAGGCTTCGCATAGCTATCCAACGGTAATACAGAGATTTCTaacaa > 1:802612/3‑71 (MQ=255) ggCTTCAATATCAGGCTTCGCATAGCTATCCAACGGt < 1:1411334/37‑1 (MQ=255) ggCTTCAATATCAGGCTTCGCATAGCTATCCAACGGt < 1:5187/37‑1 (MQ=255) ggCTTCAATATCAGGCTTCGCATAGCTATACAACGGTAATACAGAGATTTCTAACAACGTATCCGTTTTGc > 1:315425/1‑71 (MQ=255) ttCAATATCAGGCTTCGCATGGCTATCCAACGGTAATACAGAGATTTCTAACAACGTATCCGTTTTGCCAt > 1:791195/1‑71 (MQ=255) cAATATCAGGCTTCGCATAGCTATCCAACGGTAATACAGAGATTTCTAACAACGTATCCGTTTTGCCAtt > 1:967748/1‑70 (MQ=255) cAATATCAGGCTTCGCATAGCTATCCAACGGTAATACAGAGATTTCTAACAACGTATCCGTTTTGCCAtt > 1:276708/1‑70 (MQ=255) aaTATCAGGCTTCGCATAGCTATCCAACGGTAATACAGAGATTTCTaa > 1:791108/1‑48 (MQ=255) tatCAGGCTTCGCATAGCTATCCAACGGTAATACaga < 1:1053635/37‑1 (MQ=255) tatCAGGCTTCGCATAGCTATCCAACGGTAATACAGAGATTTCTAACAACGTATCCGTTTTGc < 1:1416664/63‑1 (MQ=255) tCAGGCTTCGCATAGCTATCCAACGGTAATACAGAGATTTCTAACAACGTATCCGTTTTGCCATTGATAAc > 1:109076/1‑71 (MQ=255) cGCATGGCTATCCAACGGTAATACAGAGATTTCTAACAACGTATCCGTTTTGCCATTGATAACGTTGAt < 1:959390/69‑1 (MQ=255) cGCATAGCTATCCAACGGTAATACAGAGATTTCTAACAACGTATCCGTTTTGCCATTGATAACGTTGAt < 1:675795/69‑1 (MQ=255) gCATAGCTATCCAACGGTAATACAGAGATTTCTAACAACGTATCCGTTTTGCCATTGATAACGTTGATaa < 1:539126/70‑1 (MQ=255) gCATAGCTATCCAACGGTAATACAGAGATTTCTAACAACGTATCCGTTTTGCCATTGATAACGTTGATAAc > 1:1417624/1‑71 (MQ=255) cATAGCTATCCAACGGTAATACAGAGATTTCTAACAACGTATCCGTTTTGCCATTGATAACGTTGATAAcc > 1:555147/1‑71 (MQ=255) cATAGCTATCCAACGGTAATACAGAGATTTCTAACAACGTATCCGTTTTGCCATTGATAACGTTGATAAcc < 1:1223464/71‑1 (MQ=255) cATAGCTATCCAACGGTAATACAGAGATTTCTAACAACGTATCCGTTTTGCCATTGATAACGTTGATAAcc > 1:886109/1‑71 (MQ=255) aGCTATCCAACGGTAATACAGAGATTTCTAACAACGTATCCGTTTTGCCATTGATAACGTTGATAACCTTc > 1:329649/1‑71 (MQ=255) | CAAGGAAGCCGCTTTGTCTGGCGAGCAGTCGGCCCTGATAGTTGGCTTCAATATCAGGCTTCGCATAGCTATCCAACGGTAATACAGAGATTTCTAACAACGTATCCGTTTTGCCATTGATAACGTTGATAACCTTC > W3110S.gb/3986150‑3986286 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |