| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,012,376 | G→A | 56.5% | A500T (GCC→ACC) | rbbA → | fused ribosome‑associated ATPases |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,012,376 | 0 | G | A | 56.5% | 2.9 / 26.9 | 23 | A500T (GCC→ACC) | rbbA | fused ribosome‑associated ATPases |
| Reads supporting (aligned to +/- strand): ref base G (8/2); new base A (9/4); total (17/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01 | |||||||||||
TCACTGATGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAA > W3110S.gb/4012310‑4012434 | tcACTGATGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAaccgc > 1:890898/1‑71 (MQ=255) gCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTg > 1:894102/1‑70 (MQ=255) cACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCt > 1:340224/1‑68 (MQ=255) cACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTgg > 1:84071/1‑70 (MQ=255) gCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGaa > 1:1277372/1‑69 (MQ=255) cGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAgg < 1:471703/71‑1 (MQ=255) aaaaGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCa > 1:620850/1‑70 (MQ=255) aaaaGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCAt > 1:169886/1‑71 (MQ=255) aaaGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCAtt > 1:203961/1‑71 (MQ=255) aaGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCAttt < 1:467614/71‑1 (MQ=255) gCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCg > 1:223178/1‑71 (MQ=255) gCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCg > 1:682673/1‑71 (MQ=255) gCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCGCCTa > 1:1304627/1‑71 (MQ=255) gCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTg > 1:1555503/1‑71 (MQ=255) gCAGGAACTGGTTGAGAAACGCGTAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCgg > 1:1410387/1‑71 (MQ=255) gCAGGAACTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCAttt < 1:1572104/49‑1 (MQ=255) cAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCAttt < 1:829111/48‑1 (MQ=255) cTGGTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCggcagggca < 1:654684/71‑1 (MQ=255) ggatGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCggcagggca > 1:1109834/4‑69 (MQ=255) ggTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCggcagggca > 1:1218690/1‑69 (MQ=255) ggTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCggcagggca > 1:1211537/1‑69 (MQ=255) gTTGAGAAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCagag > 1:1656875/1‑71 (MQ=255) tGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCgg < 1:751822/59‑1 (MQ=255) gagaAACGCGGAACCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCaa > 1:1692417/1‑71 (MQ=255) | TCACTGATGCACGCCGGAAAAGTGCTTGCCAGCGGTACACCGCAGGAACTGGTTGAGAAACGCGGAGCCGCCAGTCTGGAAGAGGCATTTATCGCCTATTTGCAGGAAGCGGCAGGGCAGAGCAA > W3110S.gb/4012310‑4012434 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |