| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,017,703 | T→C | 58.8% | N1175D (AAC→GAC) | rhsB ← | rhsB element core protein RshB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,017,703 | 0 | T | C | 58.8% | ‑1.5 / 16.2 | 17 | N1175D (AAC→GAC) | rhsB | rhsB element core protein RshB |
| Reads supporting (aligned to +/- strand): ref base T (3/4); new base C (6/4); total (9/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGGCAGCCGGATAAGCTGCTGCAGCTGATGCGGGTTCTCTTCATTCAGCAGGTTGCCCCATTCATCATATTCTGCGCACCACGCTGTTGCCCCTTCCGTGCTGATGAGCGCCAG > W3110S.gb/4017650‑4017763 | cGGCAGCCGGATAAGCTGCAGCAGCTGATGCGGGTTCTCTTCATTCAGCAGGTCGCCGCATTCATCATAtt < 1:428967/71‑1 (MQ=14) ggCAGCCGGATAAGCTGCTGCAGCTGATGCGGGTTCTCTTCATTCAGCAGGTTGCCCCATTCATCATATTc > 1:875478/1‑71 (MQ=39) gCAGCCGGATAAGCTGCTGCAGCTGATGCGGGTTGTCTTCATTCAGCAGGTCGccc < 1:126877/56‑1 (MQ=11) cGGATAAGCTGCTGCAGCTGATGCGGGTTCTCTTCATTCAGCAGGTCGCCCCATtcatca > 1:1243582/1‑60 (MQ=9) gctgctGCAGCTGATGCGGGTTCTCTTCATTCAGCAGGTCGGCCCATTCATCATATTCTGCGCACCACGc > 1:1533424/1‑70 (MQ=255) ctgctgCAGCTGATGCGGGTTCTCTTCATTCAGCAGGTTGCCCCATTCATCATATTCTGCGCACCACGCTg > 1:494546/1‑71 (MQ=255) gCTGATGCGGGTTCTCTTCATTCAGCAGGTTGCCCCATTCATCATATTCTGCGCACCACGCt < 1:446188/62‑1 (MQ=255) aTGCGGGTTCTCTTCATTCAGCAGGTTGCCCCATTCATCATATTCTGCGCACCACGCTGTTGCCCCtt < 1:451381/68‑1 (MQ=40) gTTCTCTTCATTCAGCAGGTCGCCCCATTCATCATATTCTGCGCACCACGCTGTTGCCCCTTCCGTGCtga > 1:975846/1‑71 (MQ=255) gTTCTCTTCATTCAGCAGGTCGCCCCATTCATCATATTCTGCGCACCACGCTGTTGCCCCTTCCGTGCtga < 1:1620853/71‑1 (MQ=255) ttCTCTTCATTCAGCAGGTTGCCCCATTCATCATATTCTGCGCACCACGCTGTTGCCCCTTCCGTGCtgat < 1:535346/71‑1 (MQ=39) tcattcaGCAGGTCGCCCCATTCATCATATTCTGCGCACCACGCTGTTGCCCCTTCCGTGCTGATGAgcgc > 1:268716/1‑71 (MQ=21) tcattcaGCAGGTCGCCCCATTCATCATATTCTGCGCACCACGCTGTTGCCCCTTCCGTGCTGATGAgc > 1:102401/1‑69 (MQ=25) cattcaGCAGGTTGCCCCATTCATCATATTCTGCGCACCACGCTGTTGCCCCTTCCGTGCTGATGAgc < 1:751609/68‑1 (MQ=35) attcaGCAGGTCGCCCCATTCATCATATTCTGCGCACCACGCTGt > 1:1473263/1‑45 (MQ=255) ttcaGCAGGTTGCCCCATTCATCATATTCTGCGCACCACGCTGTTGCCCCTTCCGTGCTGATGAGCGCCAg > 1:1611293/1‑71 (MQ=31) ttcaGCAGGTCGCCCCATTCATCATATTCTGCGcac < 1:1475913/36‑1 (MQ=255) | CGGCAGCCGGATAAGCTGCTGCAGCTGATGCGGGTTCTCTTCATTCAGCAGGTTGCCCCATTCATCATATTCTGCGCACCACGCTGTTGCCCCTTCCGTGCTGATGAGCGCCAG > W3110S.gb/4017650‑4017763 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |