Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 4,036,723 G→A 100% A184T (GCT→ACT)  ftsY → fused Signal Recognition Particle (SRP) receptor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,036,7230GA87.5% 15.4 / ‑3.2 8A184T (GCT→ACT) ftsYfused Signal Recognition Particle (SRP) receptor
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (2/5);  total (3/5)
Fisher's exact test for biased strand distribution p-value = 3.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAA  >  W3110S.gb/4036659‑4036793
                                                                |                                                                      
aGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCg                                                                        >  1:473400/1‑65 (MQ=255)
          gagGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCACTCAg                                                                   >  1:1345292/1‑60 (MQ=255)
                   aTGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCa                                                <  1:408411/70‑1 (MQ=255)
                         gtTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCAGGAAAAACCGACCAAAGAAgg                                         >  1:709187/1‑71 (MQ=255)
                                cGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAgg                                         >  1:907284/1‑64 (MQ=255)
                                      aagaagaGCAGCCGGTGGAAGAAATCACTCAGGAGCAGGaaa                                                         <  1:655264/42‑1 (MQ=255)
                                                     tGGAAGAAATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGttttt                                    <  1:1138579/48‑1 (MQ=255)
                                                          gaaATCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCTGTTaaa        <  1:745118/71‑1 (MQ=255)
                                                            aaTCACTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTCCGCGCGCCTGAAACGCAGCCTGTTaaa        <  1:228987/69‑1 (MQ=255)
                                                                gCTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCaa  <  1:150649/71‑1 (MQ=255)
                                                                |                                                                      
AGCGGCAGAAGAGGCGGTGATGGTGGTTCCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGAGCAGGAAAAACCGACCAAAGAAGGTTTTTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAA  >  W3110S.gb/4036659‑4036793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑