Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 4,083,312 G→A 45.5% G325E (GGG→GAG)  rtcB → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,083,3120GA45.5% ‑1.8 / 10.2 11G325E (GGG→GAG) rtcBconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (2/4);  new base A (2/3);  total (4/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01

CGTGCTGGTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCCGGGCGGGTAATGAGCCGAACTAA  >  W3110S.gb/4083251‑4083378
                                                             |                                                                  
cgTGCTGGTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGa                                                             <  1:453966/69‑1 (MQ=255)
       gTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCCTTATCGTCCGTGATCTGGGAAATGAagag                                                    <  1:645657/71‑1 (MQ=255)
               ggAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTg                                            >  1:1718874/1‑71 (MQ=255)
                      ttCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTGc                                     <  1:1675024/71‑1 (MQ=255)
                         ccGGTTCGAGGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTGCTGTTCGTGCAGc                                  <  1:1120197/71‑1 (MQ=255)
                            gTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCa                                <  1:410577/70‑1 (MQ=255)
                                   gggAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGcc                         <  1:861424/70‑1 (MQ=255)
                                           aaaGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCCGGGCGGGt                 >  1:855284/1‑70 (MQ=255)
                                                 ttATCGTCCGTGAGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCCGGGCGGGTAATGAGc          >  1:1528572/1‑71 (MQ=255)
                                                  tATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCCGGGCGGGTAATGa            >  1:208400/1‑68 (MQ=255)
                                                         cGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCCGGGCGGGTAATGAGCCGAACTaa  <  1:9253/71‑1 (MQ=255)
                                                             |                                                                  
CGTGCTGGTCAATATGGAATTATTCCCGGTTCGATGGGAGCAAAAAGCTTTATCGTCCGTGGGCTGGGAAATGAAGAGTCGTTCTGTTCGTGCAGCCACGGTGCCGGGCGGGTAATGAGCCGAACTAA  >  W3110S.gb/4083251‑4083378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑