| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,125,004 | A→T | 7.7% | D167V (GAT→GTT) | dam → | DNA adenine methylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,125,004 | 0 | A | T | 7.7% | 145.2 / 4.4 | 52 | D167V (GAT→GTT) | dam | DNA adenine methylase |
| Reads supporting (aligned to +/- strand): ref base A (21/27); new base T (2/2); total (23/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-01 | |||||||||||
AGCAGAGTTGTATCACTTCGCTGAAAAAGCGCAGAATGCCTTTTTCTATTGTGAGTCTTACGCCGATAGCATGGCGCGCGCAGATGATGCATCCGTCGTCTATTGCGATCCGCCTTATGCACCGCTGTCTGCGAC > W3110S.gb/4124939‑4125073 | aGCAGAGTTGTATCACTTCGCTGAAAAAGCGCAGAATGCCTTTTTCTATTGTGAGTCTTACGCCGATAGCa < 1:988715/71‑1 (MQ=255)aGCAGAGTTGTATCACTTCGCTGAAAAAGCGCAGAATGCCTTTTTCTATTGTGAGTCTTACGCCGATAGCa < 1:214166/71‑1 (MQ=255) cAGAGTTGTATCACTTCGCTGAAAAAGCGCAGAATGCCTTTTTCTATTGTGAGTCTTACGCCGATAGCATg > 1:1422269/1‑71 (MQ=255) gagTTGTATCACTTCGCTGAAAAAGCGCAGAATGCCTTTTTCTATTGTGAGTCTTACGCCGATAGCATGgc > 1:970576/1‑71 (MQ=255) gagTTGTATCACTTCGCTGAAAAAGCGCAGAATGCCTTTTTCTATTGTGAGTCTTACGCCGATAGCATGgc > 1:122689/1‑71 (MQ=255) gagTTGTATCACTTCGCTGAAAAAGCGCAGAATGCCTTTTTCTATTGTGAGTCTTACGCCGATAGCATGgc < 1:247652/71‑1 (MQ=255) gagTTGTATCACTTCGCTGAAAAAGCGCAGAATGCCTTTTTCTATTGTGAGTCTTACGCCGATAGCATGgc > 1:1623611/1‑71 (MQ=255) gTATCACTTCGCTGAAAAAGCGCAGAATGCCTTTTTCTATTGTGAGTCTTACGCCGATAGCATg > 1:1442625/1‑64 (MQ=255) tCACTTCGCTGAAAAAGCGCAGAATGCCTTTTTCTATTGTGAGTCTTACGCCGATAGCATGGCGCGCGCa < 1:80863/70‑1 (MQ=255) aCTTCGCTGAAAAAGCGCAGAATGCCTTTTTCTATTGTGAGTCTTACGCCGATAGCa > 1:242685/1‑57 (MQ=255) cTTCGCTGAAAAAGCGCAGAATGCCTTTTTCTATTGTGAGTCTTACGCCGATAGCATGGCGCGCGCAgat > 1:944759/1‑70 (MQ=255) tCGCTGAAAAAGCGCAGAATGCCTTTTTCTATTGTGAGTCTTACGCCGATAGCATGGCGCGCGCa < 1:614080/65‑1 (MQ=255) cGCTGAAAAAGCGCAGAATGCCTTTTTCTATTGTGAGTCTTACGCCGATAGCATGGCGCGCGCAgatga < 1:866728/69‑1 (MQ=255) cGCTGAAAAAGCGCAGAATGCCTTTTTCTATTGTGAGTCTTACGCCGATAGCATGGCGCGCGCAGatgatg > 1:811378/1‑71 (MQ=255) cGCTGAAAAAGCGCAGAATGCCTTTTTCTATTGTGAGTCTTACGCCGATAGCATGGCGCGCGCAGatgatg > 1:1551104/1‑71 (MQ=255) gCTGAAAAAGCGCAGAATGCCTTTTTCTATTGTGAGTCTTACGCCGATAGCATGGCGCGCGCAGATGATGc < 1:525201/71‑1 (MQ=255) gCTGAAAAAGCGCAGAATGCCTTTTTCTATTGTGAGTCTTACGCCGATAGCATGGCGCGCGCAGATGATGc < 1:364511/71‑1 (MQ=255) gCTGAAAAAGCGCAGAATGCCTTTTTCTATTGTGAGTCTTACGCCGATAGCATGGCGCGCGCAGATGATGc < 1:1026484/71‑1 (MQ=255) cTGAAAAAGCGCAGAATGCCTTTTTCTATTGTGAGTCTTACGCCGATAGCATGGCGCGCGCAGATGATGCa < 1:492047/71‑1 (MQ=255) aaaaaGCGCAGAATGCCTTTTTCTATTGTGAGTCTTACGCCGTTAGCATGGCGCGCGCAGATGATGCATcc > 1:1647/1‑71 (MQ=255) aaaGCGCAGAATGCCTTTTTCTATTGTGAGTCTTACGCCGATAGCATgg > 1:1768586/1‑49 (MQ=255) aaaGCGCAGAATGCCTTTTTCTATTGTGAGTCTTACGCCGATAGCATGGCGCGCGCAGATGATGCATCcgt > 1:911472/1‑71 (MQ=255) cgcAGAATGCCTTTTTCTATTGTGAGTCTTACGCCGATAGc < 1:1075103/41‑1 (MQ=255) cgcAGAATGCCTTTTTCTATTGTGAGTCTTACGCCGATAGc < 1:1456160/41‑1 (MQ=255) aTGCCTTTTTCTATTGTGAGTCTTACGCCGATAGCATGGCGCGCGCAGATGATGCATCcgtc < 1:1291729/62‑1 (MQ=255) aTGCCTTTTTCTATTGTGAGTCTTACGCCGATAGCATGGCGCGCGCAGATGATGCATCcgtc < 1:131841/62‑1 (MQ=255) gCCTTTTTCTATTGTGAGTCTTACGCCGATAGCATGGCGCGCGCAgat < 1:533654/48‑1 (MQ=255) ccTTTTTCTATTGTGAGTCTTACGCCGATAGCATGGCGCGCGCAGATGATGCATCCGTCGTCTATTGCGa < 1:939547/70‑1 (MQ=255) ccTTTTTCTATTGTGAGTCTTACGCCGATAGCATGGCGCGCGCAGATGATGCATCCGTCGTCTATTGCGa < 1:1518072/70‑1 (MQ=255) tttttCTATTGTGAGTCTTACGCCGTTAGCATGGCGCGCGCAgat < 1:97811/45‑1 (MQ=255) tttttCTATTGTGAGTCTTACGCCGATAGCATGGCGCGCGCAgat < 1:863045/45‑1 (MQ=255) tttttCTATTGTGAGTCTTACGCCGATAGCATGGCGCGCGCAgat < 1:1744619/45‑1 (MQ=255) tttttCTATTGTGAGTCTTACGCCGATAGCATGGCGCGCGCAGATGATGCATCcg < 1:1232236/55‑1 (MQ=255) tttttCTATTGTGAGTCTTACGCCGATAGCATGGCGCGCGCAGATGATGCATCCGTCGTCTATTGCGATcc > 1:1648617/1‑71 (MQ=255) tttCTATTGTGAGTCTTACGCCGTTAGCATGgcgcgcg > 1:1348668/1‑38 (MQ=255) tttCTATTGTGAGTCTTACGCCGATAGCATGGCGCGCGCAGATGATGCATCCGTCGTCTa > 1:396103/1‑60 (MQ=255) ttCTATTGTGAGTCTTACGCCGATAGCATGgcgcgcg < 1:681088/37‑1 (MQ=255) tCTATTGTGAGTCTTACGCCGATAGCATGGCGCGCGCAGATGATGCATCCGTCGTCTa > 1:254677/1‑58 (MQ=255) tCTATTGTGAGTCTTACGCCGATAGCATGGCGCGCGCAGATGATGCATCCGTCGTCTa > 1:548531/1‑58 (MQ=255) tCTATTGTGAGTCTTACGCCGATAGCATGGCGCGCGCAGATGATGCATCCGTCGTCTATTGCGATCCGCCt > 1:243929/1‑71 (MQ=255) aTTGTGAGTCTTACGCCGTTAGCATGGCGCGCGCAg < 1:904902/36‑1 (MQ=255) ttGTGAGTCTTACGCCGATAGCATGGCGCGCGCAGATGATGCATCCGTCGTCTATTGCGATCCGCCtt < 1:981549/68‑1 (MQ=255) gtgAGTCTTACGCCGATAGCATGGCGCGCGCAgat < 1:1510567/35‑1 (MQ=255) gtgAGTCTTACGCCGATAGCATGGCGCGCGCAGATGATGCATCCGTCGTCTATTGCGATcc < 1:611385/61‑1 (MQ=255) gtgAGTCTTACGCCGATAGCATGGCGCGCGCAGATGATGCATCCGTCGTCTATTGCGATcc < 1:826251/61‑1 (MQ=255) gtgAGTCTTACGCCGATAGCATGGCGCGCGCAGATGATGCATCCGTCGTCTATTGCGATCCGCCTTATGCa > 1:1712052/1‑71 (MQ=255) aGTCTTACGCCGATAGCATGGCGCGCGCAGATGATTCATCCGTCGTCTATTGCGATCCGCCTTATGCACCg < 1:876344/71‑1 (MQ=255) tCTTACGCCGATAGCATGGCGCGCGCAGATGATGCATCCGTCGTCTATTGCGATCCGcc < 1:403474/59‑1 (MQ=255) ttACGCCGATAGCATGGCGCGCGCAGATGATGCATCCGTCGTCTATTGCGATCCGCCTTATGCACCGCTGt > 1:508485/1‑71 (MQ=255) aCGCCGATAGCATGGCGCGCGCAGATGATGCATCCGTCGTCTATTGCGATCCGCCTTATGCACCGCTGTCt > 1:1007686/1‑71 (MQ=255) gATAGCATGGCGCGCGCAGATGATGCATCCGTCGTCTATTGCGAt > 1:1157685/1‑45 (MQ=255) gATAGCATGGCGCGCGCAGATGATGCATCCGTCGTCTATTGCGATCCGCCTTATGCACCGCTGTCTGCGAc > 1:846156/1‑71 (MQ=255) | AGCAGAGTTGTATCACTTCGCTGAAAAAGCGCAGAATGCCTTTTTCTATTGTGAGTCTTACGCCGATAGCATGGCGCGCGCAGATGATGCATCCGTCGTCTATTGCGATCCGCCTTATGCACCGCTGTCTGCGAC > W3110S.gb/4124939‑4125073 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |