| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,173,819 | G→A | 17.4% | V43M (GTG→ATG) | bfr → | bacterioferritin, iron storage and detoxification protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,173,819 | 0 | G | A | 17.4% | 53.2 / 5.1 | 23 | V43M (GTG→ATG) | bfr | bacterioferritin, iron storage and detoxification protein |
| Reads supporting (aligned to +/- strand): ref base G (10/9); new base A (2/2); total (12/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01 | |||||||||||
GTCGCAATCAATCAGTACTTTCTCCATGCCCGAATGTTTAAAAACTGGGGTCTCAAACGTCTCAATGATGTGGAGTATCATGAATCCATTGATGAGATGAAACACGCCGATCGTTATATTGAGCGCATTCTTTTTCTGG > W3110S.gb/4173750‑4173888 | gTCGCAATCAATCAGTACTTTCTCCATGCCCGAATGTTTAAAAACTGGGGTCTCAAACGTCTCAatgatg < 1:1744308/70‑1 (MQ=255) cGCAATCAATCAGTACTTTCTCCATGCCCGAATGTTTAAAAACTGGGGTCTCAAACGTCTCAATGAtgtg > 1:1075515/1‑70 (MQ=255) tACTTTCTCCATGCCCGAATGTTTAAAAACTGGGGTCTCAAACGTCTCAATGATGTGGAGTATCATGAATc < 1:1530764/71‑1 (MQ=255) tttCTCCATGCCCGAATGTTTAAAAACTGGGGTCTCAAACGTCTCAATGATGTGGAGTATCATGAATCCa < 1:439108/70‑1 (MQ=255) tctcCATGCCCGAATGTTTAAAAACTGGGGTCTCAAACGTCTCAATGATATGGAGTATCATg > 1:954189/1‑62 (MQ=255) cATGCCCGAATGTTTAAAAACTGGGGTCTCAAACGTCTCAATGATGTGGAGTATCATGAATCCATtgat < 1:296584/69‑1 (MQ=255) aTGCCCGAATGTTTAAAAACTGGGGTCTCAAACGTCTCAATGATGTGGAGTATCATGAATCCATTgatgag < 1:1586986/71‑1 (MQ=255) ccGAATGTTTAAAAACTGGGGTCTCAAACGTCTCAATGATGTGTAGTATCATGAATCCATTgatgagatga < 1:1082098/71‑1 (MQ=255) gAATGTTTAAAAACTGGGGTCTCAAACGTCTCAATGATGTGGAGTATCATGAATCCATTGATGAGATGaaa > 1:935301/1‑71 (MQ=255) gAATGTTTAAAAACTGGGGTCTCAAACGTCTCAATGATGTGGAGTATCATGAATCCATTGATGAGATGaaa > 1:154600/1‑71 (MQ=255) aaTGTTTAAAAACTGGGGTCTCAAACGTCTCAATGATGTGGAGTATCATGAATCCATTGATGAGATGaaa > 1:57677/1‑70 (MQ=255) aTGTTTAAAAACTGGGGTCTCAAACGTCTCAATGATGTGGAGTAt > 1:1201329/1‑45 (MQ=255) tGTTTAAAAACTGGGGTCTCAAACGTCTCAATGAtgtg > 1:1172253/1‑38 (MQ=255) tttAAAAACTGGTGTCTCAAACGTCTCAATGATGTGGAGTATCATGAATCCATTGATGAGATGAAACACGc > 1:437397/1‑71 (MQ=255) tttAAAAACTGGGGTCTCAAACGTCTCAATGATATGTAGTATCATGAATc < 1:1699781/50‑1 (MQ=255) tttAAAAACTGGGGTCTCAAACGTCTCAATGATATGGAGTATCATGAATc < 1:829948/50‑1 (MQ=255) tAAAAACTGGGGTCTCAAACGTCTCAATGATGTGGa > 1:1651891/1‑36 (MQ=255) tctcAAACGTCTCAATGATGTGGAGTATCATGAATc < 1:1316007/36‑1 (MQ=255) tctcAAACGTCTCAATGATATGGAGTATCATGAATCCATTGATGAGATGAAACACGCCGATCGTTATAtt > 1:1469177/1‑70 (MQ=255) cAAACGTCTCAATGATGTGGAGTATCATGAATCCATTGATGAGATGAAACACGCCGATCGt < 1:1014077/61‑1 (MQ=255) aaaCGTCTCAATGATGTGGAGTATCATGAATCCATtgatg > 1:761122/1‑40 (MQ=255) aaaCGTCTCAATGATGTGGAGTATCATGAATCCATTGATGAGATGAAACACGCCGATCGTTATATTGAgcg > 1:613684/1‑71 (MQ=255) aaaCGTCTCAATGATGTGGAATATCATGAATCCATTgatgaga > 1:878873/1‑43 (MQ=255) tcAATGATGTGGAGTATCATGAATCCATTGATGAGATGAAACACGCCGATCGTTATATTGAGCGCATTCtt > 1:868264/1‑71 (MQ=255) aaTGATGTGGAGTATCATGAATCCATTGATGAGATGAAACACGCCGATCGTTATATTGAGCGCATTCttt < 1:301806/70‑1 (MQ=255) aaTGATGTGGAGTATCATGAATCCATTGATGAGATGAAACACGCCGATCGTTATATTGAGCGCATTCttt < 1:119848/70‑1 (MQ=255) atgatgTGGAGTATCATGAATCCATTGATGAGATGAAACACGCCGATCGTTATATTGAGCGCATTCttttt < 1:32250/71‑1 (MQ=255) tgatgTGGAGTATCATGAATCCATTGATGAGATGAAACACGCCGATCGTTATAtt < 1:81713/55‑1 (MQ=255) tgtgGAGTATCATGAATCCATTGATGAGATGAAACACGCCGATCGTTATATTGAGCGCATTCTTTTTCTgg < 1:1529117/71‑1 (MQ=255) gtgGAGTATCATGAATCCATTGATGAGATGAAACACGCCGATCGTTATATTGAGCGCATTCTTTTTCTgg < 1:330535/70‑1 (MQ=255) | GTCGCAATCAATCAGTACTTTCTCCATGCCCGAATGTTTAAAAACTGGGGTCTCAAACGTCTCAATGATGTGGAGTATCATGAATCCATTGATGAGATGAAACACGCCGATCGTTATATTGAGCGCATTCTTTTTCTGG > W3110S.gb/4173750‑4173888 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |