| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,350,232 | G→A | 52.0% | R3C (CGC→TGC) | yjdF ← | conserved inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,350,232 | 0 | G | A | 52.0% | ‑4.7 / 33.6 | 25 | R3C (CGC→TGC) | yjdF | conserved inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base G (4/8); new base A (8/5); total (12/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.29e-01 | |||||||||||
CAGGATTAACGTTAGCGTCAGTGCGCTGGTGTTAAGAATTAACGGCTTGAGTGTGCGGGTCATGGTGAGTTCGCAAATCAGGGAAAATACTGACTATTCACCAGCAAGCGTGAAATTACAA > W3110S.gb/4350176‑4350296 | cAGGATTAACGTTAGCGTCAGTGCGCTGGTGTTAAGAATTAACGGCTTGAGTGTGCAGGTCATGGTGAGtt > 1:1623571/1‑71 (MQ=255)cAGGATTAAAGTTAGCGTCAGTGCGCTGGTGTTAAGAATTAACGGCTTGAGTGTGCGGGTCATGGTGAGt < 1:134300/70‑1 (MQ=255) aTTAACGTTAGCGTCAGTGCGCTGGTGTTAAGAATTAACGGCTTGAGTGTGCGGGTCATGGTGAGTTCGCa < 1:584588/71‑1 (MQ=255) ttAACGTTAGCGTCAGTGCGCTGGTGTTAAGAATTAACGGCTTGAGTGTGCAGGTCATGGTGAGTTCGCa > 1:409078/1‑70 (MQ=255) aaCGTTAGCGTCAGTGCGCTGGTGTTAAGAATTAACGGCTTGAGTGTGCAGGTCATGGTGAGTTCGCAAAt > 1:751856/1‑71 (MQ=255) aaCGTTAGCGTCAGTGCGCTGGTGTTAAGAATTAACGGCTTGAGTGTGCAGGTCATGGTGAGTTCGCAAAt > 1:204823/1‑71 (MQ=255) aCGTTAGCGTCAGTGCGCTGGTGTTAAGAATTAACGGCTTGAGTGTGCAGGTCATGGTGAGTTCGCAAATc < 1:992912/71‑1 (MQ=255) cGTTAGCGTCAGTGCGCTGGTGTTAAGAATTAACGGCTTGAGTGTGCGGGTCATGGTGAGTTCGCa < 1:741176/66‑1 (MQ=255) aGCGTCAGTGCGCTGGTGTTAAGAATTAACGGCTTGAGTGTGCagg > 1:802036/1‑46 (MQ=255) gCGTCAGTGCGCTGGTGTTAAGAATTAACGGCTTGAGTGTGCAGGTCATGGTGAGTTCGCAAATCAGGGa > 1:1072377/1‑70 (MQ=255) gTCAGTGCGCTGGTGTTAAGAATTAACGGCTTGAGTGTGCGGGTCATGGTGAGTTCGCAAATCAGGGaaaa < 1:777623/71‑1 (MQ=255) tCAGTGCGCTGGTGTTAAGAATTAACGGCTTGAGTGTGCAGGTCATGGTGAGTTCGCAAATCAGGGaaaa < 1:979503/70‑1 (MQ=255) tGCGCTGGTGTTAAGAATTAACGGCTTGAGTGTGCAGGTCATGGTGAGTTCGCAAATc < 1:228607/58‑1 (MQ=255) tGCGCTGGTGTTAAGAATTAACGGCTTGAGTGTGCAGGTCATGGTGAGTTCGCAAATc < 1:508227/58‑1 (MQ=255) gcTGGTGTTAAGAATTAACGGCTTGAGTGTGCGGGTCATGGTGAGTTCGCAAATCAGGGAAAATACTGAc > 1:698249/1‑70 (MQ=255) gtgtTAAGAATTAACGGCTTGAGTGTGCGGGTCATGGTGAGTTCGCAAATCAGGGAAAATACTGACTAtt < 1:1148467/70‑1 (MQ=255) ttAAGAATTAACGGCTTGAGTGTGCGGGTCATGGTg < 1:755022/36‑1 (MQ=255) ttAAGAATTAACGGCTTGAGTGTGCGGGTCATGGTGAGTTCGCAAATCAGGGAAAATACTGAc < 1:1364310/63‑1 (MQ=255) ttAAGAATTAACGGCTTGAGTGTGCGGGTCATGGTGAGTTCGCAAATCAGGGAAAATACTGAc < 1:766196/63‑1 (MQ=255) aaGAATTAACGGCTTGAGTGTGCGGGTCATGGTGa > 1:1546183/1‑35 (MQ=255) aaGAATTAACGGCTTGAGTGTGCGGGTCATGGTGAGTTCGCAAATCAGGGAAAATACTGACTATTCACCAg > 1:1272841/1‑71 (MQ=255) aaTTAACGGCTTGAGTGTGCAGGTCATGGTGAGTTCGCAAATCAGGGaaa < 1:560224/50‑1 (MQ=255) aaCGGCTTGAGTGTGCAGGTCATGGTGAGTTCGCAAATCAGGGAAAATACTGACTATTCACCAGCAAGCg > 1:247819/1‑70 (MQ=255) aaCGGCTTGAGTGTGCAGGTCATGGTGAGTTCGCAAATCAGGGAAAATACTGACTATTCACCAGCAAGCGt > 1:1440394/1‑71 (MQ=255) gCTTGAGTGTGCGGGTCATGGTGAGTTCGCAAATCAGGGAAAATa > 1:1443272/1‑45 (MQ=255) tgCAGGTCATGGTGAGTTCGCAAATCAGGGAAAATACTGACTATTCACCAGCAAGCGTGAAATTACaa > 1:295329/1‑68 (MQ=255) | CAGGATTAACGTTAGCGTCAGTGCGCTGGTGTTAAGAATTAACGGCTTGAGTGTGCGGGTCATGGTGAGTTCGCAAATCAGGGAAAATACTGACTATTCACCAGCAAGCGTGAAATTACAA > W3110S.gb/4350176‑4350296 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |