| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,392,090 | G→T | 43.7% | L655M (CTG→ATG) | yjeP ← | predicted mechanosensitive channel |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,392,090 | 0 | G | T | 43.7% | 13.2 / 12.1 | 16 | L655M (CTG→ATG) | yjeP | predicted mechanosensitive channel |
| Reads supporting (aligned to +/- strand): ref base G (4/5); new base T (3/4); total (7/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATCATCATGTTCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAG > W3110S.gb/4392025‑4392154 | aaTCATCATGTTCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGaa < 1:91146/71‑1 (MQ=255) atGTTCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGc > 1:633534/1‑71 (MQ=255) tatGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCt > 1:1664657/1‑70 (MQ=255) tggttggtAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTGa < 1:801467/70‑1 (MQ=255) tggttggtAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTGAg < 1:1061000/71‑1 (MQ=255) tggttggtAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGc < 1:916284/45‑1 (MQ=255) tggttggtAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGa < 1:1478982/70‑1 (MQ=255) gtAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTGa < 1:1274351/64‑1 (MQ=255) aaTGTTGTCGCCGCTGCCCTCTTTGTTGAGATACATCGGAATCCCGGCCTTTTTCAGGCTGAgg > 1:1050650/1‑64 (MQ=255) aTGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGaccac > 1:1739683/1‑71 (MQ=255) ttgtCGCCGCTGCCCTCTTTGTTGAGATACATCGGaa > 1:1169376/1‑37 (MQ=255) ttgtCGCCGCTGCCCTCTTTGTTGAGATACAGAGGAATCCCGGCCTTTTTCAGGCTGa > 1:1150619/1‑58 (MQ=255) ctTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGt < 1:446049/46‑1 (MQ=255) ctTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGt < 1:926276/46‑1 (MQ=255) tttGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAAt > 1:288454/1‑69 (MQ=255) gaTACATCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATaaag < 1:1657424/71‑1 (MQ=255) | AATCATCATGTTCCACAGCATATGGTTGGTAATGTTGTCGCCGCTGCCCTCTTTGTTGAGATACAGCGGAATCCCGGCCTTTTTCAGGCTGAGGGTGACCACCGCCAGCGCACCGCAAATGAGGATAAAG > W3110S.gb/4392025‑4392154 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |