Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 4,407,692 C→T 100% R325C (CGT→TGT)  hflK → modulator for HflB protease specific for phage lambda cII repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb4,407,6920CT83.3% 22.2 / ‑0.1 12R325C (CGT→TGT) hflKmodulator for HflB protease specific for phage lambda cII repressor
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (6/4);  total (7/5)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATA  >  W3110S.gb/4407648‑4407755
                                            |                                                               
aaCTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATGGaaaaa                                       <  1:1578980/71‑1 (MQ=255)
                  aaGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCgaga                                                  >  1:1495017/1‑42 (MQ=255)
                  aaGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCg                      >  1:95925/1‑70 (MQ=255)
                   aGCCGCGCCGGAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCa                    <  1:257479/71‑1 (MQ=255)
                            ggAAATTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTgg           <  1:13324/71‑1 (MQ=255)
                              aaaTTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCa                    <  1:883386/60‑1 (MQ=255)
                              aaaTTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGtt         <  1:949846/71‑1 (MQ=255)
                               aaTTACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGtt         >  1:1000127/1‑70 (MQ=255)
                                  tACTCGCGAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAAc      >  1:16661/1‑70 (MQ=255)
                                   aCTCGCGAGCGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGa    >  1:1264954/1‑71 (MQ=255)
                                      cgcgAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATa  >  1:619523/1‑70 (MQ=255)
                                      cgcgAGTGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATa  >  1:979468/1‑70 (MQ=255)
                                            |                                                               
AACTTCTGCCGGAATATAAAGCCGCGCCGGAAATTACTCGCGAGCGTCTGTATATCGAGACGATGGAAAAAGTGTTGGGTAACACCCGCAAAGTGCTGGTTAACGATA  >  W3110S.gb/4407648‑4407755

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑