| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,443,410 | G→A | 40.0% | L179L (CTG→TTG) | ytfJ ← | predicted transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,443,410 | 0 | G | A | 40.0% | 10.2 / 19.2 | 20 | L179L (CTG→TTG) | ytfJ | predicted transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (6/6); new base A (6/2); total (12/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01 | |||||||||||
ATAGATAACGATAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCACG > W3110S.gb/4443340‑4443471 | aTAGATAACGATAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTgcag < 1:1051658/71‑1 (MQ=255) aGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAACAGGTCCATCAc > 1:500962/1‑71 (MQ=255) aaaTTATTTTTGCAGAATTTATTTTATTGCCATTATTTGAGCAACTTTTGCAACAGGTCCATc < 1:117806/63‑1 (MQ=255) aaaTTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAACAGGTCCATc < 1:1433622/63‑1 (MQ=255) aaaTTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAACAGGTCCATCACTtgctg > 1:666782/1‑71 (MQ=255) aaTTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAACAGGTCCATCACTtgctg > 1:78454/1‑70 (MQ=255) ttattTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCAc < 1:1466877/71‑1 (MQ=255) tattTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAACAGGTCCATCAc > 1:1382617/1‑61 (MQ=255) aaTTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGgtgt > 1:196892/1‑71 (MQ=255) ttATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACtt < 1:652483/49‑1 (MQ=255) aTGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTtgc > 1:195004/1‑49 (MQ=255) gTTATTGCCATTATTTGAGCAACTTTTGCAACAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGcc > 1:384078/1‑71 (MQ=255) tGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTtg > 1:1125793/1‑41 (MQ=255) tGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTtg > 1:368418/1‑41 (MQ=255) tGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTtg > 1:1127744/1‑41 (MQ=255) gCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTc < 1:1512017/70‑1 (MQ=255) gCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCt > 1:1165833/1‑71 (MQ=255) ccATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCAc < 1:1672733/45‑1 (MQ=255) aGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTg < 1:485730/71‑1 (MQ=255) aGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTg < 1:1297055/71‑1 (MQ=255) aCTTTTGCAACAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCAcg > 1:1130319/1‑71 (MQ=255) | ATAGATAACGATAGGGGAAAATTATTTTTGCAGAATTTATGTTATTGCCATTATTTGAGCAACTTTTGCAGCAGGTCCATCACTTGCTGCACCTCTTCCGGTGTGAGCGCCCCGTCTTTGGCCCATTGCACG > W3110S.gb/4443340‑4443471 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |