| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,477,260 | G→A | 54.1% | intergenic (‑45/+236) | pyrL ← / ← yjgH | pyrBI operon leader peptide/predicted mRNA endoribonuclease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,477,260 | 0 | G | A | 54.1% | ‑1.2 / 27.9 | 23 | intergenic (‑45/+236) | pyrL/yjgH | pyrBI operon leader peptide/predicted mRNA endoribonuclease |
| Reads supporting (aligned to +/- strand): ref base G (3/7); new base A (4/8); total (7/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGT > W3110S.gb/4477197‑4477325 | gTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGgcgc > 1:53119/1‑70 (MQ=255) tCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCa < 1:964811/70‑1 (MQ=255) cTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAacggcg < 1:252305/59‑1 (MQ=255) tACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCAt < 1:1145297/58‑1 (MQ=255) tACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATAtt < 1:1624025/71‑1 (MQ=255) tcctccCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGt < 1:883204/71‑1 (MQ=255) gCAAATTGTCCGGCATTATACTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCaa < 1:1518938/71‑1 (MQ=255) aaaaTTGTCCGGCATTATACTCATCGTCAGAATCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCaaa < 1:506030/70‑1 (MQ=255) aaaTTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGa > 1:1161768/1‑48 (MQ=255) aaaTTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAAc > 1:401591/1‑71 (MQ=255) cGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTAt < 1:194923/70‑1 (MQ=255) ttATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGAGCAAACGGTTTAttt < 1:904934/67‑1 (MQ=255) ttATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGGCATCGCAAACGGTTTAttt < 1:4053/67‑1 (MQ=255) ataCTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAAt > 1:1305610/1‑69 (MQ=255) taCTCATCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTaa < 1:963273/71‑1 (MQ=255) tCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCa < 1:1761905/49‑1 (MQ=255) tCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCa < 1:1052517/49‑1 (MQ=255) tCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAg > 1:439541/1‑71 (MQ=255) aTCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACgg > 1:1044644/1‑52 (MQ=255) tCGTCAGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTa < 1:790682/64‑1 (MQ=255) aGAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGt < 1:1374395/70‑1 (MQ=255) gAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTaaaa > 1:471014/1‑61 (MQ=255) gAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTa < 1:1124429/58‑1 (MQ=255) gAAACGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTa < 1:1079863/58‑1 (MQ=255) | GTCGAACACACTGAACCATACATCCTCCCGGCAAATTGTCCGGCATTATACTCATCGTCAGAAGCGGCGCAAGCATTTGATGCAATATTTTGTCAGCGCAAACGGTTTATTTGAATTAAAAGTCAAGGT > W3110S.gb/4477197‑4477325 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |