| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,561,090 | G→A | 55.6% | intergenic (‑88/‑585) | yjiC ← / → yjiD | hypothetical protein/DNA replication/recombination/repair protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,561,090 | 0 | G | A | 55.6% | 4.2 / 33.0 | 27 | intergenic (‑88/‑585) | yjiC/yjiD | hypothetical protein/DNA replication/recombination/repair protein |
| Reads supporting (aligned to +/- strand): ref base G (7/5); new base A (11/4); total (18/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGAAACACTCTA > W3110S.gb/4561022‑4561152 | tATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGAcatgca > 1:74824/1‑71 (MQ=255) tCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGacatacat > 1:759344/1‑68 (MQ=255) tCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGtt > 1:721856/1‑71 (MQ=255) tCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGtt > 1:1050426/1‑71 (MQ=255) cTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGttt < 1:186686/69‑1 (MQ=255) tGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCtg > 1:1001368/1‑71 (MQ=255) aTGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGTTTCtg < 1:1345779/69‑1 (MQ=255) tGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCtgttg < 1:960084/71‑1 (MQ=255) aGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGt < 1:1568637/70‑1 (MQ=255) gATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAAct > 1:155896/1‑71 (MQ=255) aTTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGTTTCtgtt > 1:1497135/1‑51 (MQ=255) tagtaAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGatat > 1:194602/1‑71 (MQ=255) tagtaAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGatat > 1:58653/1‑71 (MQ=255) tagtaAAAAGAGAAGTAAACAAATGACATACATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGatat > 1:375595/1‑71 (MQ=255) taAAAAGAGAAGTAAACAAATGACATACATGTTTCtgttg < 1:264167/40‑1 (MQ=255) taAAAAGAGAAGTAAACAAATGACATACATGTTTCtgtt > 1:917602/1‑39 (MQ=255) agagAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTa < 1:677223/65‑1 (MQ=255) agaAGTAAACAAATGACATACATGTTTCTGTTGTa > 1:1732428/1‑35 (MQ=255) tAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAActc < 1:1168992/43‑1 (MQ=255) aaCAAATGACATGCATGTTTCTGTTGTAGTGGTATCAACTCTACACGGTGATATTAAAGGGTAGGAAaca < 1:316373/70‑1 (MQ=255) aaCAAATGACATACATGTTTCTGTTGTAGTGATATCAACTCTACACgg > 1:1474406/1‑48 (MQ=255) aaCAAATGACATACATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGa > 1:1631972/1‑51 (MQ=255) aaTGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGAAACACTCTa < 1:1086334/71‑1 (MQ=255) aaTGACATACATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGAGACActct > 1:618233/1‑70 (MQ=255) gACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAgg > 1:1381696/1‑53 (MQ=255) gACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAgg > 1:674525/1‑53 (MQ=255) gACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAgg > 1:1735292/1‑53 (MQ=255) gACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAgg > 1:1683905/1‑53 (MQ=255) gACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAgg > 1:749963/1‑53 (MQ=255) atgcatgTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGt > 1:1597621/1‑52 (MQ=255) tgcatgTTTCTGTTGTAGTGATATCAACTCTACACGGTGata > 1:770884/1‑42 (MQ=255) | TATTTCACTGATGTTTAGAAAAATAGATAAATTTTTCTGTAGTAAAAAGAGAAGTAAACAAATGACATGCATGTTTCTGTTGTAGTGATATCAACTCTACACGGTGATATTAAAGGGTAGGAAACACTCTA > W3110S.gb/4561022‑4561152 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |