| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,563,589 | T→A | 100% | K207M (AAG→ATG) | iadA ← | isoaspartyl dipeptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,563,589 | 0 | T | A | 100.0% | 60.1 / NA | 22 | K207M (AAG→ATG) | iadA | isoaspartyl dipeptidase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (16/6); total (16/6) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGG > W3110S.gb/4563522‑4563654 | ggTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCattt < 1:591942/71‑1 (MQ=255) gcagcTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTa < 1:310603/65‑1 (MQ=255) cTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCa < 1:1406087/71‑1 (MQ=255) cGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca > 1:547670/1‑71 (MQ=255) cGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAaca > 1:351582/1‑71 (MQ=255) ggCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAacac < 1:376091/71‑1 (MQ=255) cAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACg > 1:1455062/1‑63 (MQ=255) ttttCCAGCAGGTCATAAATAGGCTGTAACGCCatttt < 1:1059088/38‑1 (MQ=255) ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAacac > 1:383089/1‑57 (MQ=255) ttCCAGCAGGTCATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACg > 1:722237/1‑58 (MQ=255) cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCAtgtg < 1:188704/40‑1 (MQ=255) cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAg > 1:144586/1‑70 (MQ=255) cATAAATAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAg > 1:449876/1‑70 (MQ=255) aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGaa > 1:1469442/1‑39 (MQ=255) aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGaa > 1:343875/1‑39 (MQ=255) aaTAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAAc > 1:624520/1‑71 (MQ=255) tAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGcaa > 1:643001/1‑48 (MQ=255) tAGGCTGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCa > 1:712985/1‑50 (MQ=255) tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCa > 1:1451267/1‑45 (MQ=255) tGTAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAAc > 1:995074/1‑71 (MQ=255) tAACGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAg > 1:1352055/1‑44 (MQ=255) aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCaa > 1:14814/1‑58 (MQ=255) aCGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCgg < 1:1269468/71‑1 (MQ=255) cGCCATTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGtt < 1:415246/44‑1 (MQ=255) | GGTCGGCAGCAGCTTGCTGATCGGCACATCGCAGTTTTCCAGCAGGTCATAAATAGGCTGTAACGCCTTTTTACTGTCGCCCATGTGGAACACGGTGACGCCAGGTTTACCGCCGAGCAAACCGCCAACGCGG > W3110S.gb/4563522‑4563654 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |