| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,580,803 | C→T | 55.6% | pseudogene (1987/2721 nt) | yjiV → | ECK4334:JW5954:b4486; conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,580,803 | 0 | C | T | 55.6% | 0.0 / 20.9 | 18 | pseudogene (1987/2721 nt) | yjiV | ECK4334:JW5954:b4486; conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (3/5); new base T (7/3); total (10/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01 | |||||||||||
ATGGGGGTTGATACCGATCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACGGTAGAACTGAACG > W3110S.gb/4580737‑4580869 | aTGGGGGTTGATACCGATCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGa < 1:1265764/71‑1 (MQ=255) ggTTGATACCGATCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGc < 1:63003/71‑1 (MQ=255) gTTGATAGCGATCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCa > 1:34559/1‑71 (MQ=255) aCCGATCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCACCTGaa > 1:764551/1‑71 (MQ=255) ccGATCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCACCTGAAt < 1:1189644/71‑1 (MQ=255) tCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACg < 1:1313924/71‑1 (MQ=255) atgaCGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCACCTGAATGACGAACtggt > 1:1295797/1‑71 (MQ=255) gaCGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACtggt > 1:1333673/1‑69 (MQ=255) gCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCACCTGAATGACGaa > 1:1059394/1‑61 (MQ=255) gCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCACCTGAATGACGaa > 1:336103/1‑61 (MQ=255) gCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGAAc < 1:1317573/58‑1 (MQ=255) gCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGAAc < 1:1760610/58‑1 (MQ=255) tctcGCAGCCAGCAGGCAACGATTCCGGGGTCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGtct > 1:1250517/1‑71 (MQ=255) ctcGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGaa > 1:1351004/1‑53 (MQ=255) aaCGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACGGTAg < 1:1250923/71‑1 (MQ=255) ttCCGGGGTCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACGGTAGAACt > 1:978484/1‑70 (MQ=255) ccGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACGGTAGAACTGaa > 1:1719268/1‑71 (MQ=255) gggCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACGGTAGAACTGAACg < 1:51396/70‑1 (MQ=255) | ATGGGGGTTGATACCGATCGCGTGATGACGCTGGCCTCTCGCAGCCAGCAGGCAACGATTCCGGGGCCGGAGTGGCACCTGAATGACGAACTGGTGGTGCGTAGTCTCGGTTATAAAACGGTAGAACTGAACG > W3110S.gb/4580737‑4580869 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |