| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,586,637 | G→A | 72.2% | P365L (CCG→CTG) | hsdM ← | DNA methylase M |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,586,637 | 0 | G | A | 72.2% | 21.9 / 9.8 | 18 | P365L (CCG→CTG) | hsdM | DNA methylase M |
| Reads supporting (aligned to +/- strand): ref base G (2/3); new base A (3/10); total (5/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.83e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
CATATTGGTACGCAGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGTCTTCACGCCCTGAGCGTAAAAAATACCGG > W3110S.gb/4586579‑4586706 | cATATTGGTACGCAGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCAGGTTCg < 1:1670774/65‑1 (MQ=255) tatTGGTACGCAGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCAGGTTCGcc > 1:137355/1‑65 (MQ=255) ttGGTACGCAGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCggg > 1:1664830/1‑57 (MQ=255) tACGCAGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCAGGTTCGCCACCGTCCCTTTg < 1:1517926/71‑1 (MQ=255) aGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCAGGTTCGCCAcc < 1:1013915/57‑1 (MQ=255) aGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCAGGTTCGCCAcc < 1:815024/57‑1 (MQ=255) cacCCACACATCATCGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCa > 1:1678622/1‑71 (MQ=255) cacacaTCATCGGTACAGTTCTTATCCTGATTCAGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGtt > 1:1216535/1‑71 (MQ=255) cacaTCATCGGTACAGTTCTTATCCTGATTCAGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTgg < 1:276603/71‑1 (MQ=255) atcGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGTCTTCa > 1:211122/1‑71 (MQ=255) gTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTAGTAAAGAACAGCACGTTGGTCtt < 1:1474537/65‑1 (MQ=255) gTACAGTTCTTATCCTGATTCAGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGTCTTCACGcc < 1:557218/71‑1 (MQ=255) tACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTgg < 1:534586/42‑1 (MQ=255) cAGTTCTTATCCTGATTCAGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGTCTTCACGCCCTg < 1:826592/71‑1 (MQ=255) aGTTCTTATCCTGATTCAGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGTCTTCACGCCCTGa < 1:8495/71‑1 (MQ=255) cTTATCCTGATTCAGGTTCGCCACCGTCCCTTTGGTaaa > 1:990367/1‑39 (MQ=255) cTGATTCAGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGTCTTCACGCCCTGAGCGTaaaaaa < 1:797308/71‑1 (MQ=255) aTTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGTCTTCACGCCCt < 1:910932/56‑1 (MQ=255) aTTCAGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGTCTTCACGCCCTGAGCGTAAAAAATAc < 1:271339/71‑1 (MQ=255) gggTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGTCTTCACGCCCTGAGCGTAAAAAATACCgg < 1:1252036/70‑1 (MQ=255) | CATATTGGTACGCAGGTCATACACCCACACATCATCGGTACAGTTCTTATCCTGATTCGGGTTCGCCACCGTCCCTTTGGTAAAGAACAGCACGTTGGTCTTCACGCCCTGAGCGTAAAAAATACCGG > W3110S.gb/4586579‑4586706 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |