| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,588,402 | C→T | 52.0% | L1032L (CTG→CTA) | hsdR ← | endonuclease R |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,588,402 | 0 | C | T | 52.0% | ‑6.5 / 34.4 | 25 | L1032L (CTG→CTA) | hsdR | endonuclease R |
| Reads supporting (aligned to +/- strand): ref base C (4/8); new base T (9/4); total (13/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.14e-01 | |||||||||||
CGGGTGAGATCGCGCGGGCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACCAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTCACC > W3110S.gb/4588334‑4588471 | cGGGTGAGATCGCGCGGGCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACCa < 1:1379227/70‑1 (MQ=255) gTGAGATCGCGCGGGCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACCAGc > 1:1123373/1‑69 (MQ=255) aataaCTGCCTGCAATGCCGGTTGCGCGTTCGGCGAACGTAGCACTAGCGAGTCAAACGCTTCGAGGa > 1:1570362/1‑68 (MQ=255) tgcctgcAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTgcg > 1:264974/1‑71 (MQ=255) tgcctgcAATGCCGGTTGCGCGTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTgcg > 1:1745297/1‑71 (MQ=255) gCCGGTTGCGCGTTCGGGGAACGTTGCACCAGCGAGTCAAAGGCTTc > 1:160357/1‑47 (MQ=255) gTTGCGCGTTCGGGGAACGTTGCACCAGCGAGTCAAAGGCTTCGAGGAAATCCTgcggc < 1:529231/59‑1 (MQ=255) gTTGCGCGTTCGGGGAACGTTGCACCAGCGAGTCAAAGGCTTCGAGGAAATCCTgcggc < 1:18462/59‑1 (MQ=255) cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCg < 1:55158/70‑1 (MQ=255) cgcgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCg < 1:1340823/70‑1 (MQ=255) cgcgTTCGGGGAACGTTGCACCAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCg > 1:504522/1‑70 (MQ=255) gcgTTCGGGGAACGTTGCACCAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTa < 1:615332/71‑1 (MQ=255) gcgTTCGGGGAACGTTGCACCAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTa < 1:286384/71‑1 (MQ=255) gcgTTCGGGGAACGTTGCACCAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTa < 1:1653109/71‑1 (MQ=255) cgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTc < 1:887002/58‑1 (MQ=255) cgTTCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTa > 1:1626001/1‑70 (MQ=255) ttCGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGt > 1:458559/1‑58 (MQ=255) tCGGGGAACGTTGCACCAGCGAGTCAAAGGCTTCGAGGAAATc > 1:852429/1‑43 (MQ=255) cGGGGAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAAt > 1:1121062/1‑59 (MQ=255) ggggAACGTTGCACCAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGa < 1:186840/71‑1 (MQ=255) gAACGTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGAttt < 1:1089920/71‑1 (MQ=255) cGTTGCACCAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTac < 1:1504112/71‑1 (MQ=255) gTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTa > 1:655744/1‑49 (MQ=255) gTTGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAAt > 1:1245459/1‑51 (MQ=255) tGCACTAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACt > 1:1391644/1‑71 (MQ=255) ccAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTcacc < 1:1541033/71‑1 (MQ=255) | CGGGTGAGATCGCGCGGGCGATTAATAACTGCCTGCAATGCCGGTTGCGCGTTCGGGGAACGTTGCACCAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTCACC > W3110S.gb/4588334‑4588471 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |