| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,632,877 | A→G | 37.5% | Y294C (TAC→TGC) | nadR → | bifunctional DNA‑binding transcriptional repressor and NMN adenylyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,632,877 | 0 | A | G | 37.5% | 15.9 / 22.5 | 24 | Y294C (TAC→TGC) | nadR | bifunctional DNA‑binding transcriptional repressor and NMN adenylyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (8/7); new base G (4/5); total (12/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACT > W3110S.gb/4632809‑4632941 | ccTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATAc < 1:1104823/70‑1 (MQ=255) atgaGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCg > 1:361957/1‑71 (MQ=255) atgaGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACAt > 1:775913/1‑61 (MQ=255) atgaGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGc < 1:1369230/70‑1 (MQ=255) cATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAatat < 1:1213922/71‑1 (MQ=255) gCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGcc > 1:625207/1‑70 (MQ=255) cAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCa < 1:667072/70‑1 (MQ=255) cAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCa < 1:1667893/70‑1 (MQ=255) tATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATaa < 1:558867/71‑1 (MQ=255) ttCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGaaa > 1:557982/1‑58 (MQ=255) cTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGAtt > 1:1434476/1‑45 (MQ=255) tGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGa < 1:89570/53‑1 (MQ=255) tGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGaaa > 1:1631581/1‑55 (MQ=255) gACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTgg > 1:579079/1‑70 (MQ=255) aCTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGc < 1:382298/70‑1 (MQ=255) gATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATa < 1:966344/58‑1 (MQ=255) tAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATaaa > 1:1633356/1‑58 (MQ=255) tAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATaaa > 1:956/1‑58 (MQ=255) aaaaTCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTgg > 1:496074/1‑61 (MQ=255) gcgcTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCACTTTATTGGCAtat < 1:1083208/60‑3 (MQ=25) gcgcTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGc > 1:1458178/1‑56 (MQ=255) gCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGt > 1:143175/1‑70 (MQ=255) gCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTc < 1:716406/71‑1 (MQ=255) cTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACt < 1:895410/71‑1 (MQ=255) | CCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACT > W3110S.gb/4632809‑4632941 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |