| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 251,041 | G→A | 25.9% | A48A (GCG→GCA) | dinB → | DNA polymerase IV |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 251,041 | 0 | G | A | 25.9% | 45.8 / 13.7 | 27 | A48A (GCG→GCA) | dinB | DNA polymerase IV |
| Reads supporting (aligned to +/- strand): ref base G (8/12); new base A (3/4); total (11/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
TCCCTATTGCTATTGGCGGCAGCCGCGAACGTCGGGGGGTGATCAGCACCGCCAATTATCCCGCGCGTAAATTTGGCGTACGTAGCGCTATGCCGACAGGGATGGCGCTCAAATTATGCCCACATCT > W3110S.gb/250977‑251103 | tCCCTATTGCTATTGGCGGCAGCCGCGAACGTCGGGGGGTGATCAGCACCGCCAATTATCCCGCACGTaa < 1:1410284/70‑1 (MQ=255) ttgctattgGCGGCAGCCGCGAACGTCGGGGGGTGATCAGCACCGCCAATTATCCCGCGCGTAAAt > 1:558658/1‑66 (MQ=255) ttgctattgGCGGCAGCCGCGAACGTCGGGGGGTGATCAGCACCGCCAATTATCCCGCACGTAAATTTg < 1:624014/69‑1 (MQ=255) gctattgGCGGCAGCCGCGAACGTCGGGGGGTGATCAGCACCGCCAATTATCCCGCGCGTAAATTTGGcg > 1:1520350/1‑70 (MQ=255) ggcggcAGCCGCGAACGTCGGGGGGTGATCAGCACCGCCAATTATCCCGCGCGTAAATTTGGCGTACGTAg > 1:481870/1‑71 (MQ=255) ggcAGCCGCGAACGTCGGGGGGTGATCAGCACCGCCAATTATCCCGCACGTAAATTTGGCGTACGTAgcgc < 1:800192/71‑1 (MQ=255) cGTCGGGGGGTGATCAGCACCGCCAATTATCCCGCGCGTAAATTTGGCGTACGTAGCGCTATGCCGACAgg < 1:1141042/71‑1 (MQ=255) tCGGGGGGTGATCAGCACCGCCAATTATCCCGCGCGTAAATTTgg < 1:1249130/45‑1 (MQ=255) cGGGGGGTGATCAGCACCGCCAATTATCCCGCGCGTAAATTTGGCGTACGTAGCGCTATGCCGACAGGGAt > 1:804443/1‑71 (MQ=255) ggggTGATCAGCACCGCCAATTATCCCGCGCGTAAATTTGGCGTACGTAGCGCTATGCCGACa < 1:1116052/63‑1 (MQ=255) ggggTGATCAGCACCGCCAATTATCCCGCGCGTAAATTTGGCGTACGTAGCGCTATGCCGACa < 1:101868/63‑1 (MQ=255) ggTGATCAGCACCGCCAATTATCCCGCGCGTAAATTTGGcgtacg < 1:1627697/45‑1 (MQ=255) tGATCAGCACCGCCAATTATCCCGCGCGTAAATTTGGCGTACGTAGCGCTATGCCGACAgg < 1:624288/61‑1 (MQ=255) tGATCAGCACCGCCAATTATCCCGCGCGTAAATTTGGCGTACGTAGCGCTATGCCGACAgg < 1:1186550/61‑1 (MQ=255) tGATCAGCACCGCCAATTATCCCGCACGTAAATTTGGCGTACGTAgcgc < 1:1737502/49‑1 (MQ=255) aTCAGCACCGCCAATTATCCCGCGCGTAAATTTGGCGTACGTAGCGCTATGCCGa < 1:143767/55‑1 (MQ=255) aTCAGCACCGCCAATTATCCCGCGCGTAAATTTGGCGTACGTAGCGCTATGCCGa < 1:1734810/55‑1 (MQ=255) aTCAGCACCGCCAATTATCCCGCGCGTAAATTTGGCGTACGTAGCGCTATGCCGa < 1:693129/55‑1 (MQ=255) aGCACCGCCAATTATCCCGCACGTAAATTTGGCGTACGTAGCGCTATGCCGACAGGGATGGCGCTCaaa > 1:1553435/1‑69 (MQ=255) gCCAATTATCCCGCGCGTAAATTTGGCGTACGTAGCGCTa > 1:594301/1‑40 (MQ=255) ttATCCCGCGCGTAAATTTGGCGTACGTAGCGCTATGCCGAc < 1:273188/42‑1 (MQ=255) ttATCCCGCGCGTAAATTTGGCGTACGTAGCGCTATGCCGACAGGGATGGCGCTCAAATTa > 1:1201055/1‑61 (MQ=255) aTCCCGCGCGTAAATTTGGCGTACGTAGCGCTATGCCGACAGGGATGGCGCTCAAATTATGCCCACAtct > 1:1286967/1‑70 (MQ=255) aTCCCGCGCGTAAATTTGGCGTACGTAGCGCTATGCCGACAGGGATGGCGCTCAAATTATGCCCACAtct > 1:1101217/1‑70 (MQ=255) aTCCCGCACGTAAATTTGGCGTACGTAGCGCTATGCCGACAGGGATg > 1:1356581/1‑47 (MQ=255) aTCCCGCACGTAAATTTGGCGTACGTAGCGCTATGCCGACAGGGATg > 1:655815/1‑47 (MQ=255) ccGCGCGTAAATTTGGCGTACGTAGCGCTATGCCGACAGGGATGGCGCTCAAATTATGCCCACAtc < 1:1646064/66‑1 (MQ=255) | TCCCTATTGCTATTGGCGGCAGCCGCGAACGTCGGGGGGTGATCAGCACCGCCAATTATCCCGCGCGTAAATTTGGCGTACGTAGCGCTATGCCGACAGGGATGGCGCTCAAATTATGCCCACATCT > W3110S.gb/250977‑251103 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |