| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 257,873 | A→G | 53.3% | K15K (AAA→AAG) | crl → | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 257,873 | 0 | A | G | 53.3% | ‑1.8 / 16.7 | 15 | K15K (AAA→AAG) | crl | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (5/2); new base G (5/3); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATT > W3110S.gb/257812‑257937 | cacaACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCa > 1:121814/1‑71 (MQ=255) acaacaGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCa > 1:1566438/1‑70 (MQ=255) gagaTAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAGGccc > 1:1370086/1‑71 (MQ=255) aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTc > 1:716875/1‑71 (MQ=255) aaTGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTc > 1:1738599/1‑71 (MQ=255) ttACCGAGTGGACACCCGAAGAGCAGATTGATCaaaaaa < 1:99850/39‑1 (MQ=255) ttACCGAGTGGACACCCGAAGAGCAGATTGATCaaaaaa < 1:749584/39‑1 (MQ=255) ttACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGTATATTCGTg < 1:1492129/67‑1 (MQ=255) aCCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGTATATTCGTGAAGGt < 1:1066492/70‑1 (MQ=255) aCCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGTATATTCGTGAAGGt > 1:756759/1‑70 (MQ=255) ccGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTa < 1:1184722/70‑1 (MQ=255) ccGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTa < 1:1440374/70‑1 (MQ=255) cGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAg > 1:890938/1‑71 (MQ=255) cGAAGAGCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGTatat > 1:1673600/1‑46 (MQ=255) cGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGtata > 1:509632/1‑45 (MQ=255) gCAGATTGATCAAAAAGTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTc > 1:1347902/1‑71 (MQ=255) gATTGATCAAAAAGTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCttt < 1:1381031/71‑1 (MQ=255) aaaaaaTTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGAtt < 1:401244/70‑1 (MQ=255) | CACAACAGGAGATAGCAATGACGTTACCGAGTGGACACCCGAAGAGCAGATTGATCAAAAAATTTACCGCACTAGGCCCGTATATTCGTGAAGGTAAGTGCAAAGATAATCGATTCTTTTTCGATT > W3110S.gb/257812‑257937 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |