Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 267,502 G→T 27.8% P228H (CCT→CAT)  ykfA ← predicted GTP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb267,5020GT27.8% 52.8 / 9.6 25P228H (CCT→CAT) ykfApredicted GTP‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (5/13);  new base T (5/2);  total (10/15)
Fisher's exact test for biased strand distribution p-value = 7.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

CGGCCCCGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCGGCCATCA  >  W3110S.gb/267437‑267571
                                                                 |                                                                     
cGGCCCCGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATgg                                                                  <  1:1432684/71‑1 (MQ=255)
cGGCCCCGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATg                                                                   >  1:826741/1‑70 (MQ=255)
     ccGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTg                                                             >  1:1242191/1‑71 (MQ=255)
           gTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGc                                                            <  1:619/66‑1 (MQ=255)
             ttCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGAcacca                                                     >  1:860911/1‑71 (MQ=255)
                aCCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCg                                                           >  1:1130311/1‑62 (MQ=255)
                aCCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGACACCACCg                                                  >  1:1061487/1‑71 (MQ=255)
                 ccAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCg                                                  <  1:310244/70‑1 (MQ=255)
                   aaaaTCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGACACCACCGGGc                                               <  1:1479577/71‑1 (MQ=255)
                            aCGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGcc                                       >  1:31990/1‑70 (MQ=255)
                             cGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTc                                     <  1:1308093/71‑1 (MQ=255)
                               aGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCAc                                   <  1:1106208/71‑1 (MQ=255)
                                    gTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTg                                >  1:1092093/1‑69 (MQ=255)
                                     ttCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTc                                     <  1:26951/63‑1 (MQ=255)
                                     ttCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggca                             <  1:850540/71‑1 (MQ=255)
                                     ttCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTgg                               <  1:348712/69‑1 (MQ=255)
                                             cTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTa                     <  1:502281/71‑1 (MQ=255)
                                                ccGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAatc                  <  1:646165/71‑1 (MQ=255)
                                                 cgtcgtGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAatca                 <  1:1593726/71‑1 (MQ=255)
                                                   tcgtGCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAatcat                >  1:1344559/1‑70 (MQ=255)
                                                   tcgtGCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAatcat                >  1:1342622/1‑70 (MQ=255)
                                                        cGAAAGGAAGGATGGAGTTGCGACCCCACCGGGCTGGTGGCCTCAAGTGGCAGGCAc                        >  1:1418608/1‑57 (MQ=255)
                                                         gAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCt                                      <  1:278161/42‑1 (MQ=255)
                                                         gAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCt                                      <  1:481536/42‑1 (MQ=255)
                                                         gAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCt                                      <  1:479590/42‑1 (MQ=255)
                                                           aaggaaggATGGAGTTGCGACACCACCGGGCTGGTGGCCTCa                                    >  1:338619/1‑42 (MQ=255)
                                                              gaatgaTGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCGGCCAt    >  1:948265/1‑71 (MQ=255)
                                                                aggaTGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCGGCCATCa  <  1:316890/71‑1 (MQ=255)
                                                                aggaTGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCGGCCATCa  <  1:1758971/71‑1 (MQ=255)
                                                                 |                                                                     
CGGCCCCGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCGGCCATCA  >  W3110S.gb/267437‑267571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: