| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 267,502 | G→T | 27.8% | P228H (CCT→CAT) | ykfA ← | predicted GTP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 267,502 | 0 | G | T | 27.8% | 52.8 / 9.6 | 25 | P228H (CCT→CAT) | ykfA | predicted GTP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (5/13); new base T (5/2); total (10/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
CGGCCCCGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCGGCCATCA > W3110S.gb/267437‑267571 | cGGCCCCGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATgg < 1:1432684/71‑1 (MQ=255)cGGCCCCGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATg > 1:826741/1‑70 (MQ=255) ccGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTg > 1:1242191/1‑71 (MQ=255) gTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGc < 1:619/66‑1 (MQ=255) ttCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGAcacca > 1:860911/1‑71 (MQ=255) aCCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCg > 1:1130311/1‑62 (MQ=255) aCCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGACACCACCg > 1:1061487/1‑71 (MQ=255) ccAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCg < 1:310244/70‑1 (MQ=255) aaaaTCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGACACCACCGGGc < 1:1479577/71‑1 (MQ=255) aCGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGcc > 1:31990/1‑70 (MQ=255) cGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTc < 1:1308093/71‑1 (MQ=255) aGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCAc < 1:1106208/71‑1 (MQ=255) gTTCCCGGACTACCGTCGTGCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTg > 1:1092093/1‑69 (MQ=255) ttCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTc < 1:26951/63‑1 (MQ=255) ttCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTggca < 1:850540/71‑1 (MQ=255) ttCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTgg < 1:348712/69‑1 (MQ=255) cTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTa < 1:502281/71‑1 (MQ=255) ccGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAatc < 1:646165/71‑1 (MQ=255) cgtcgtGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAatca < 1:1593726/71‑1 (MQ=255) tcgtGCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAatcat > 1:1344559/1‑70 (MQ=255) tcgtGCGAAAGGAATGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAatcat > 1:1342622/1‑70 (MQ=255) cGAAAGGAAGGATGGAGTTGCGACCCCACCGGGCTGGTGGCCTCAAGTGGCAGGCAc > 1:1418608/1‑57 (MQ=255) gAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCt < 1:278161/42‑1 (MQ=255) gAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCt < 1:481536/42‑1 (MQ=255) gAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCt < 1:479590/42‑1 (MQ=255) aaggaaggATGGAGTTGCGACACCACCGGGCTGGTGGCCTCa > 1:338619/1‑42 (MQ=255) gaatgaTGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCGGCCAt > 1:948265/1‑71 (MQ=255) aggaTGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCGGCCATCa < 1:316890/71‑1 (MQ=255) aggaTGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCGGCCATCa < 1:1758971/71‑1 (MQ=255) | CGGCCCCGACGGTTTCACCAAAATCGCTACGAGCCTGTTCCCGGACTACCGTCGTGCGAAAGGAAGGATGGAGTTGCGACACCACCGGGCTGGTGGCCTCACGTGGCAGGCACTTAATCATCCGCTCGGCCATCA > W3110S.gb/267437‑267571 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |