| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 275,785 | G→A | 50.5% | V413I (GTA→ATA) | mmuP → | predicted S‑methylmethionine transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 275,785 | 0 | G | A | 50.5% | ‑5.1 / 25.3 | 20 | V413I (GTA→ATA) | mmuP | predicted S‑methylmethionine transporter |
| Reads supporting (aligned to +/- strand): ref base G (4/5); new base A (4/6); total (8/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01 | |||||||||||
CAACAAGGTAAGGCATTGAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAGCGCAGAG > W3110S.gb/275725‑275846 | caacaaGGTAAGGCATTGAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTCTTAGGt < 1:2143/69‑1 (MQ=255) aaGGTAAGGCATTGAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTtgtg > 1:947785/1‑71 (MQ=255) aGGCATTGAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGGGCCAATATTAGGTTTtgtgctgtgc < 1:1629986/71‑1 (MQ=255) gAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGCCtggtgg > 1:1615331/1‑71 (MQ=255) gTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGTGCTGTGCCTGGTGGcc > 1:28856/1‑71 (MQ=255) aTTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTtgtgctgt < 1:576291/56‑1 (MQ=255) aCATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGtt < 1:170865/70‑1 (MQ=255) cATTATCGCGCGCCGTGGTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGtt < 1:1625601/69‑1 (MQ=255) ccGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGa < 1:740104/71‑1 (MQ=255) ccGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGa < 1:695805/71‑1 (MQ=255) tGGTATCCGCTGGTGCCAATATTAGGTTTtgtgctgtg < 1:999995/38‑1 (MQ=255) gTATCCGCTGGTGCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAg > 1:1107925/1‑71 (MQ=255) tCCGCTGGTGCCAGTATTAGGTTTTGTGCTGTGCCt < 1:196457/36‑1 (MQ=255) cGCTGGTGCCAGTATTAGGTTTTGTGCTGTGCCtgg > 1:352772/1‑36 (MQ=255) cGCTGGTGCCAGTATTAGGTTTTGTGCTGTGCCTGGTGGCCtgtg > 1:940201/1‑45 (MQ=255) cGCTGGTGCCAATATTAGGTTTTGTGCTGTGCCtgg > 1:1222474/1‑36 (MQ=255) cTGGTGCCATTATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAGCGCagag < 1:376881/71‑1 (MQ=255) cTGGTGCCAATATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTgg < 1:651370/52‑1 (MQ=255) ggTGCCAGTATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCg > 1:813423/1‑56 (MQ=255) gTGCCAGTATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAg < 1:1274887/61‑1 (MQ=255) gTATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGc < 1:809338/40‑1 (MQ=255) | CAACAAGGTAAGGCATTGAGTGAATTACATTATCGCGCGCCGTGGTATCCGCTGGTGCCAGTATTAGGTTTTGTGCTGTGCCTGGTGGCCTGTGTTGGGCTGGCATTCGATCCAGCGCAGAG > W3110S.gb/275725‑275846 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |