| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 285,498 | G→A | 38.1% | A294T (GCC→ACC) | yagG → | predicted sugar transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 285,498 | 0 | G | A | 38.1% | 15.7 / 17.2 | 21 | A294T (GCC→ACC) | yagG | predicted sugar transporter |
| Reads supporting (aligned to +/- strand): ref base G (10/3); new base A (4/4); total (14/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGCCTCGCCACCATGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCG > W3110S.gb/285432‑285560 | aGCCTCGCCACCATGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCtt > 1:952813/1‑71 (MQ=255) gCCACCATGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTg > 1:752411/1‑71 (MQ=255) caTGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGatca < 1:208122/71‑1 (MQ=255) aTGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGatcat > 1:498533/1‑71 (MQ=255) tCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGatca < 1:1002479/66‑1 (MQ=255) gCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCAtcgtc > 1:1624476/1‑64 (MQ=255) tGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCt > 1:1027092/1‑71 (MQ=255) cACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCaa > 1:330281/1‑40 (MQ=255) cACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCaa > 1:679944/1‑40 (MQ=255) cACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGc > 1:375255/1‑71 (MQ=255) aCGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAg > 1:951944/1‑69 (MQ=255) cGCCTGCTGGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTg < 1:166738/63‑1 (MQ=255) tgctgGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTc < 1:453447/55‑1 (MQ=255) ctgGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGc > 1:88323/1‑55 (MQ=255) ctgGGCCGCTTCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGa < 1:316671/70‑1 (MQ=255) gggCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGa > 1:120950/1‑56 (MQ=255) ccGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTc > 1:1209764/1‑70 (MQ=255) tCGACCGCGTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGa > 1:1439702/1‑60 (MQ=255) ccGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCctgct < 1:137666/54‑1 (MQ=255) cgcgTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCcggc > 1:583017/1‑71 (MQ=255) gcgTCACCACCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCcggcg < 1:1426252/71‑1 (MQ=255) | AGCCTCGCCACCATGTTCGGCTCGCTTTGCTCCTCACGCCTGCTGGGCCGCTTCGACCGCGTCACCGCCTTCAAGTGGATCATCGTCGCCTACTCGCTGATCAGCCTGCTGATTTTCGTCACCCCGGCG > W3110S.gb/285432‑285560 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |