| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 429,850 | T→C | 70.6% | Y8H (TAT→CAT) | yajD → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 429,850 | 0 | T | C | 70.6% | 22.1 / 8.0 | 17 | Y8H (TAT→CAT) | yajD | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (3/2); new base C (4/8); total (7/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCG > W3110S.gb/429784‑429914 | ttGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACCATgc > 1:898274/1‑71 (MQ=255) gAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATGATCCCAAAAAACCATGCGCgg > 1:1690446/1‑71 (MQ=255) gAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACCATGCGCgg < 1:927161/71‑1 (MQ=255) gcgcGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGa > 1:535553/1‑70 (MQ=255) cgTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGc < 1:433222/71‑1 (MQ=255) aCCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGc < 1:999876/70‑1 (MQ=255) tACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATcg > 1:1606122/1‑71 (MQ=255) tttCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGa > 1:692226/1‑70 (MQ=255) cAGGTAACTTTTCATGGCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCAt > 1:1615357/1‑71 (MQ=255) ggCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGt > 1:272879/1‑71 (MQ=255) gCTATCATCCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGt < 1:992966/70‑1 (MQ=255) atcatcCCAAAAAACCATGCGCGGTTAGAAAGCGGc < 1:1556111/36‑1 (MQ=255) atcatcCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTa < 1:574392/62‑1 (MQ=255) tcatcCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGaaa < 1:1031671/45‑1 (MQ=255) atcCCAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCt < 1:858661/70‑1 (MQ=255) ccAAAAAACCATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATcc < 1:1115306/59‑1 (MQ=255) aaaaaCTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATAAAAAATCTATCCGTGGGTCTGCGGTCg < 1:46054/71‑1 (MQ=255) | TTGGGAAACTGCGCGTAACCCTACATTTCATCCAGGTAACTTTTCATGGCTATCATCCCAAAAAACTATGCGCGGTTAGAAAGCGGCTATCGCGAAAAAGCATTAAAAATCTATCCGTGGGTCTGCGGTCG > W3110S.gb/429784‑429914 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |