| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 478,343 | C→T | 47.6% | A45T (GCT→ACT) | ylaC ← | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 478,343 | 0 | C | T | 47.6% | ‑2.3 / 26.8 | 21 | A45T (GCT→ACT) | ylaC | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (3/8); new base T (5/5); total (8/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.17e-01 | |||||||||||
CCAGTAGCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGT > W3110S.gb/478277‑478404 | ccAGTAGCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGTAACg < 1:1390614/71‑1 (MQ=255)ccAGTAGCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACg > 1:1613986/1‑71 (MQ=255) aGCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGTAACGTACa > 1:494908/1‑70 (MQ=255) gCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACAt < 1:384305/70‑1 (MQ=255) ccAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATAc < 1:1317336/71‑1 (MQ=255) acagaGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGTAACGt < 1:1082396/61‑1 (MQ=255) acagaGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGTAACGTACATACCGa > 1:1544521/1‑70 (MQ=255) acagaGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGAt < 1:843656/71‑1 (MQ=255) cagaGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGTAACGTACATACCGATa < 1:904085/71‑1 (MQ=255) ggACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAAc > 1:719740/1‑39 (MQ=255) cAACATCACCGCCAGGGTGGCAAAAAAAGTAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATa > 1:1741016/1‑70 (MQ=255) cAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATaaa > 1:1569965/1‑48 (MQ=255) aCATCACCGCCAGGGTGGCAAAAAAAGTAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATaaaa < 1:695384/71‑1 (MQ=255) cAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTa < 1:1740823/70‑1 (MQ=255) cAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGt < 1:1337937/71‑1 (MQ=255) aaaaaaGTAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGa < 1:1404281/55‑1 (MQ=255) aaaaaaGCAACGTACATACCGATAAACAGCCCCGGATGtt < 1:979056/40‑1 (MQ=255) aaaaaaGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAg < 1:1724951/63‑1 (MQ=255) aaaaaaGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCggg < 1:1213170/67‑1 (MQ=255) aaaGTAACGTACATACCGATAAACAGCCCCGGATGatt > 1:1614967/1‑38 (MQ=38) aaaGTAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCg > 1:1352680/1‑62 (MQ=255) | CCAGTAGCCAGACAGAGCCTGACAGCGTTTCGGACTGCAACATCACCGCCAGGGTGGCAAAAAAAGCAACGTACATACCGATAAACAGCCCCGGATGTTTACGGATAAAACTGATACTAAAGCGGGGT > W3110S.gb/478277‑478404 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |