| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 527,659 | A→G | 65.2% | K163E (AAA→GAA) | ylbH → | conserved hypothetical protein, rhs‑like |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 527,659 | 0 | A | G | 65.2% | 23.0 / 17.8 | 23 | K163E (AAA→GAA) | ylbH | conserved hypothetical protein, rhs‑like |
| Reads supporting (aligned to +/- strand): ref base A (5/3); new base G (7/8); total (12/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTTTGTCGAGTGTCTAAATTAAATGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTACGGCAGAAAAGTAAAGCTATCC > W3110S.gb/527592‑527721 | aTTTTGTCGAGTGTCTAAATTAAATGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGCTATGAAAAag < 1:1598066/71‑1 (MQ=255) ttttGTCGAGTGTCTAAATTAAATGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAagaaga < 1:338736/71‑1 (MQ=255) gAGTGTCTAAATTAAATGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAagagatta > 1:19643/1‑69 (MQ=255) tgtCTAAATTAAATGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAagagattagagat > 1:798247/1‑71 (MQ=255) tCTAAATTAAATGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATgaagaa > 1:333602/1‑57 (MQ=255) taaattaaatGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACg > 1:367046/1‑71 (MQ=255) aattaaatGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAagagattaga < 1:1617899/62‑1 (MQ=255) ttaaatGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAagagattagagat < 1:686583/63‑1 (MQ=255) aatGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTaaa > 1:466686/1‑71 (MQ=255) aCGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAAgagattagagatta < 1:1452336/58‑1 (MQ=255) aCGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAAgagattagagatta < 1:1589265/58‑1 (MQ=255) aCGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAaga < 1:1136444/46‑1 (MQ=255) tGGTGTTTGCCGATCGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATCTGTTCg > 1:812278/1‑71 (MQ=255) ggTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTCgg > 1:157036/1‑71 (MQ=255) ttAGCCGATCGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATg < 1:1038763/71‑1 (MQ=255) aGCCGATCGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGt < 1:329027/66‑1 (MQ=255) aGCCGATCGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGt < 1:351745/66‑1 (MQ=255) aGCCGATCGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGt < 1:1093765/66‑1 (MQ=255) cGATCCGCGAAAGGGCTGGGTTATGAAAAagagattagagat > 1:1501696/1‑42 (MQ=255) cGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTaaa > 1:1202528/1‑49 (MQ=255) cGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTaaa > 1:64109/1‑49 (MQ=255) cGGCGAAAGGGCTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTACGGCAg > 1:294835/1‑70 (MQ=255) aGGGCTGGGTTATGAAAAAGAGA‑TAGAGATTACGGGTTAAATCTGTTCGGTATGTACGGCAGAAAAGTa > 1:1560180/1‑69 (MQ=255) cTGGGTTATGAAGAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTACGGCAGAAAAGTAAAGCt < 1:1147014/71‑1 (MQ=255) gTTATGAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTACGGCAGAAAAGTAAAGCTATcc > 1:503230/1‑71 (MQ=255) aaaaaGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTACGGCAGAAAAg > 1:1566863/1‑54 (MQ=255) | ATTTTGTCGAGTGTCTAAATTAAATGACGCTGGTGTTAGCCGATCGGCGAAAGGGCTGGGTTATGAAAAAGAGATTAGAGATTACGGGTTAAATCTGTTCGGTATGTACGGCAGAAAAGTAAAGCTATCC > W3110S.gb/527592‑527721 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |