| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 530,077 | T→C | 55.0% | Y125C (TAT→TGT) | ybbB ← | tRNA 2‑selenouridine synthase, selenophosphate‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 530,077 | 0 | T | C | 55.0% | 3.4 / 22.1 | 20 | Y125C (TAT→TGT) | ybbB | tRNA 2‑selenouridine synthase, selenophosphate‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (4/5); new base C (6/5); total (10/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGT > W3110S.gb/530010‑530139 | aaTCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTACAAc > 1:1356031/1‑71 (MQ=255) aTCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTccac > 1:774984/1‑71 (MQ=255) aTCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTccac > 1:664428/1‑71 (MQ=255) tCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTACAACCGCCTTccacc < 1:983925/71‑1 (MQ=255) tttGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTACAACCGCCTTCCACCAGCGGa > 1:527342/1‑71 (MQ=255) ttGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTACAACCGCCTTCCACCAGCGGa < 1:57896/70‑1 (MQ=255) aaTAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTACAACCGCCTTCCACCAGCGGATAATCAATcccc < 1:1587016/70‑1 (MQ=255) tAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTtat < 1:81039/36‑1 (MQ=255) tAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTACAACCGCCTTCCACCAGCGGATAATCAATCCCCGCt > 1:1101007/1‑71 (MQ=255) tCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCg < 1:942119/38‑1 (MQ=255) tCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATaat < 1:1274922/57‑1 (MQ=255) gCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCtgcatg > 1:1324409/1‑71 (MQ=255) gCCTGAATCGCGGTCTGGCGCAGTGCCTTACAACCGCCTTCCACCAGc > 1:1028308/1‑48 (MQ=255) gCCTGAATCGCGGTCTGGCGCAGTGCCTTACAACCGCCTTCCACCAGCGGa > 1:1285778/1‑51 (MQ=255) tGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCaa > 1:1283951/1‑71 (MQ=255) tGAATCGCGGTCTGGCGCAGTGCCTTACAACCGCCTTCCACCAGCGGATaatca < 1:512688/54‑1 (MQ=255) cgGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCtt < 1:651068/71‑1 (MQ=255) ggTCTGGCGCAGTGCATTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCttt < 1:1270419/71‑1 (MQ=255) gTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCttt < 1:745494/70‑1 (MQ=255) gcAGTGCCTTACAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAata < 1:452506/71‑1 (MQ=255) gCCTTACAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATAtgt > 1:1611924/1‑69 (MQ=255) tataACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAata < 1:1712723/62‑1 (MQ=255) | AATCAGCACTATCGGTTTTTGTGCCAGTTCAATAGTCGCCTGAATCGCGGTCTGGCGCAGTGCCTTATAACCGCCTTCCACCAGCGGATAATCAATCCCCGCTGCATGCAACCAGCTTTGCACAATATGT > W3110S.gb/530010‑530139 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |