| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 548,801 | G→A | 54.5% | pseudogene (1221/1259 nt) pseudogene (964/1002 nt) |
ylbE → ylbE → |
ECK0512:JW0508+JW0507:b4572; hypothetical protein ECK0512:JW0508:b0519; hypothetical protein, C‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 548,801 | 0 | G | A | 54.5% | ‑2.5 / 27.5 | 22 | pseudogene (1221/1259 nt) pseudogene (964/1002 nt) | ylbE ylbE | ECK0512:JW0508+JW0507:b4572; hypothetical protein ECK0512:JW0508:b0519; hypothetical protein, C‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base G (6/4); new base A (7/5); total (13/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.39e-01 | |||||||||||
GGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCT > W3110S.gb/548743‑548867 | ggggATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCa > 1:1369960/1‑70 (MQ=255) ggATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTg > 1:1650049/1‑71 (MQ=255) ttGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATg > 1:569715/1‑71 (MQ=255) gTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAg > 1:781645/1‑70 (MQ=255) gCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGaa > 1:1293453/1‑71 (MQ=255) cGGGCACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAAc < 1:1486892/71‑1 (MQ=255) cGGGCACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAAc < 1:1654485/71‑1 (MQ=255) cACCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAAcgcg > 1:1308450/1‑70 (MQ=255) aCCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACgcgc > 1:1720488/1‑70 (MQ=255) aCCGCTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACgcgc > 1:214583/1‑70 (MQ=255) gCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAAt > 1:975598/1‑70 (MQ=255) cTGGCGTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGa < 1:662504/71‑1 (MQ=255) gTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGatc < 1:1309246/71‑1 (MQ=255) gTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGatc < 1:834546/71‑1 (MQ=255) gTGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGat < 1:1091761/70‑1 (MQ=255) tGCTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGa < 1:584621/54‑1 (MQ=255) gCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGa > 1:581505/1‑64 (MQ=255) cTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGatcatc > 1:336712/1‑71 (MQ=255) cTTTGAACAGACGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGatcatc > 1:499625/1‑71 (MQ=255) tGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATcatcc < 1:247058/69‑1 (MQ=255) tGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATcatcc < 1:1050933/69‑1 (MQ=255) aGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGAACATGCATCct > 1:199350/1‑69 (MQ=255) | GGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCT > W3110S.gb/548743‑548867 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |