Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 620,339 A→G 42.9% W25R (TGG→CGG)  fepG ← iron‑enterobactin transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb620,3390AG42.9% ‑0.8 / 14.0 14W25R (TGG→CGG) fepGiron‑enterobactin transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (6/2);  new base G (4/2);  total (10/4)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.98e-01

TCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGCGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATC  >  W3110S.gb/620282‑620409
                                                         |                                                                      
tCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAAc                                                            >  1:1355055/1‑70 (MQ=255)
           aaTACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGCGGa                                                <  1:1263473/71‑1 (MQ=255)
                cTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACca                                           <  1:1362920/71‑1 (MQ=255)
                  gCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACcag                                          >  1:244535/1‑70 (MQ=255)
                        tttCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAgg                                                    <  1:1157535/54‑1 (MQ=255)
                          tCCAGCGTGACGGCACCGCTGCGTAATCCCCGGATACCTGCAACCACACAGGCGGAAACCAGCAGCaaa                                   >  1:1661981/1‑69 (MQ=255)
                          tCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGCGGAAACCAGCAGCaaa                                   >  1:886843/1‑69 (MQ=255)
                            cAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGCGGAAACCAGCAGCAAACAgg                               >  1:397884/1‑71 (MQ=255)
                              gCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGCGGAAACCAGCAGCaaa                                   >  1:1225623/1‑65 (MQ=255)
                              gCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGCGGAAACCAGCAGCaaa                                   >  1:337229/1‑65 (MQ=255)
                              gCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGCGGAAACCAGCAGCAAACAGGt                              >  1:1535657/1‑70 (MQ=255)
                                                gTAATCCACGGATACCTGCAACCACACAGGCGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCga              >  1:234841/1‑68 (MQ=255)
                                                     ccccAGATACCTGCAACCACACAGGCGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGt       >  1:1607397/1‑70 (MQ=255)
                                                      cccGGATACCTGCAACCACACAGGCGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTaa     <  1:1576972/71‑1 (MQ=255)
                                                         aGATACCTGCAACCACACAGGCGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATc  <  1:876604/71‑1 (MQ=255)
                                                         |                                                                      
TCAGCGCGGCGAATACCTGCGAGGTTTCCAGCGTGACGGCACCGCTGCGTAATCCCCAGATACCTGCAACCACACAGGCGGAAACCAGCAGCAAACAGGTGATGAGTAATCGGCGAGAGACGTAAATC  >  W3110S.gb/620282‑620409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑